Keywords

  • AIDS diagnostics
  • biological engineering
  • biosensor
  • biosensors & biocontrol engr
  • DNA and RNA detection
  • microfluidic
  • nanofabrication
  • nanosensor
  • pathogen detection

Baeumner, Antje J

Professor
My research interests focus on the field of biotechnology, with special emphasis on analytical biotechnology, whereas my teaching will cover selected aspects of the entire field of biotechnology. In analytical biotechnology, chemical and biological techniques are combined and integrated into innovatively engineered devices for the detection of hazardous biological and chemical substances in the environment, in food, and in medical diagnostics. I am developing electrochemical and optical biosensors, e.g., for the direct extra-laboratory detection of pathogenic microorganisms, pesticides and natural toxins. Molecular biological techniques are also involved in the development of these biosensors for the detection of pathogens such as Cryptosporidium parvum. Microfabrication techniques are currently used for the preparation of signal transducers, and microfluidic devices (µTAS, micro Total Analytical Systems) will play a major role in the development of novel biosensing systems that will not only serve as easy-to-use sensing devices but will also allow their incorporation into more complex automated systems.

research

research and scholarship focus

  • The research is focused on the development of bioanalytical detection tools. Research is in part applied, i.e. focusing on solving a specific problem important to the safety of humans or animals, and for clinical diagnostics. This includes research toward the design and prototyping of sensors for the use in resource-limited settings, such as Sub-Saharan Africa. Other parts are involved with more basic research toward the development of bioanalytical tools. Biochemical, microbiological and nanofabrication research is being used to reach these goals.
  • My research interests focus on the field of analytical bioengineering and biotechnology working at the integration of biological and microfabricated systems. My teaching covers the molecular and cellular bioengineering aspects of biotechnology and the field of biosensors and bioanalytical techniques. In analytical biotechnology, chemical and biological techniques are combined and integrated into innovatively engineered devices for the detection of hazardous biological and chemical substances in the environment, in food, and in medical diagnostics. I am developing electrochemical and optical biosensors, e.g., for the direct extra-laboratory detection of pathogenic microorganisms, pesticides and natural toxins. Molecular biological techniques are also involved in the development of these biosensors for the detection of pathogens such as such as Cryptosporidium parvum, Dengue virus, E. coli and HIV. Microfabrication techniques are currently used for the preparation of signal transducers, and microfluidic devices (µTAS, micro Total Analytical Systems) will play a major role in the development of novel biosensing systems that will not only serve as easy-to-use sensing devices but will also allow their incorporation into more complex automated systems.

research areas

affiliations

head of

faculty appointment in

member of graduate field

administrative responsibilities

Graduate Program Director

teaching

teaching focus

In general, instruction is mainly focused on BEE 260 and BEE 459/659. In addition, emphasis is put on educating undergraduate students in research via BEE 499, 497 and 495. Also, students are encouraged to apply for fellowships such as the CoE ELI program, Hughes Scholar Program, CALS undergraduate student fellowships etc.

teaches

service

outreach focus

Outreach is broadly used such as assisting NYS-based companies in research development, presenting to alumni, seniors, high school students, assisting the local Science Museum etc. This has also been continued in part during the sabbatical stay by discussing academic research, study abroad and similar topics with students at the University of Dortmund and the Technical University of Braunschweig, Germany.

background

educational background

  • Ph.D., Universität Stuttgart: Technical Biochemistry, 1997
  • M.S., Technische Universität Braunschweig: Biotechnology, 1994

professional background

  • Associate Professor, Cornell University: Biological and Environmental Engineering, (11/04 - present)
  • Assistant Professor, Cornell University: Agricultural and Biological Engineering, (7/99 - 10/04)
  • Postdoctoral Fellow, Cornell University: Food Science &Technology, (7/97 - 6/99)

awards and distinctions

  • Reinhard Böhm Award (Braunschweig University, FRG) for independent research, 1993
  • Daimler-Benz Award for research at Cambridge University, UK, 1994
  • 2nd Workshop on Biosensors and Biological Techniques, Lund, Sweden
  • DAAD Fellowship for research at Cornell University, USA, 1997
  • Cornell College of Ag. & Life Sciences Inaugural Award for Excellence in Mentoring Undergraduate Students in Independent Research, 2001
  • Cornell College of Engineering Excellence in Teaching Award, 2002
  • Cornell College of Engineering Excellence in Teaching Award, 2006
  • Elected Vice-Co Chair for Gordon Research Conference "Bioanalytical Sensors", 2006

publications

selected publications (listing in progress)

  • Nugen, S.R., Leonard, B., Baeumner, A.J. “Application of a unique server-based oligonucleotide probe selection tool toward a novel biosensor for the detection of Streptococcus pyogenes” Biosensors and Bioelectronics (available online, 11/29/2006))
  • Katie A. Edwards, Antje J. Baeumner “Synthesis of a liposome incorporated 1-carboxyalkylxanthine-phospholipid conjugate and its recognition by an RNA aptamer” Talanta, vol 71(1) pp. 365 – 372 (2007), doi:10.1016/j.talanta.2006.04.031
  • Edwards, K.A. and Baeumner, A.J. “Optimization of DNA-tagged Dye- Encapsulating Liposomes for Lateral-Flow Assays Based on Sandwich-Hybridization” Analytical and Bioanalytical Chemistry vol. 386 (5), pp. 1335 – 1343 (2006) (http://dx.doi.org/10.1007/s00216-006-0705-x)
  • Edwards, K.A. and Baeumner, A.J. “Optimization of DNA-tagged Liposomes for Use in Microtiter Plate Analyses” Analytical and Bioanalytical Chemistry, vol. 386 (6), pp. 1613 – 1623 (2006) (doi: 10.1007/s00216-006-0743-4)
  • Li, Dapeng; Frey, Margaret W.; Baeumner, Antje J. Electrospun polylactic acid nanofiber membranes as substrates for biosensor assemblies. Journal of Membrane Science (2006), 279(1-2), 354-363.
  • Vasiliy N. Goral, Natalya V. Zaytseva, Antje J. Baeumner “Electrochemical microfluidic biosensor for the detection of nucleic acid sequences” Lab-on-Chip, vol 6(3), pp. 414 - 421 (2006) DOI 10.1039/b513239h
  • Katie A. Edwards and Antje J. Baeumner “A Sequential Injection Analysis System for the Sandwich-Hybridization-Based Detection of Nucleic Acids” Analytical Chemistry,vol. 78 (6), pp. 1958-1966 (2006)
  • Sylvia Kwakye, Vasiliy N. Goral and Antje J. Baeumner “Electrochemical microfluidic biosensor for nucleic acid detection with integrated minipotentiostat.” Biosensors and Bioelectronics, vol. 21, pp. 2217 – 223 (2006)
  • Kevin P. Nichols, Julia R. Ferullo, Antje J. Baeumner “Recirculating Microfluidic Mixer” Lab-on-Chip, vol. 6(2) pp. 242 - 246 (2006)
  • Katie A. Edwards, Harriet A. Clancy and Antje J. Baeumner “Bacillus anthracis: Toxicology, Epidemiology and Current Detection Methods” Analytical and Bioanalytical Chemistry., vol. 384, pp. 73-84 (2006)
  • Katie A. Edwards and Antje J. Baeumner “Analysis of Liposomes” Talanta, vol. 68 (5) pp. 1432-1441 (2006)
  • Katie A. Edwards and Antje J. Baeumner “Liposomes in Analysis” Talanta, vol. 68 (5) pp. 1421-1431 (2006)
  • Natalya V. Zaytseva, Richard A. Montagna, and Antje J. Baeumner “Microfluidic biosensor for the serotype-specific detection of Dengue virus” Analytical Chemistry, vol. 77, p. 7520 – 7527 (2005)
  • Natalya V. Zaytseva, Vasiliy N. Goral, Richard A. Montagna, and Antje J. Baeumner “Development of a microfluidic biosensor module for pathogen detection” Lab-on-chip, vol 5 (8), pp. 805 – 811 (2005) (issue cover picture)
  • Hsiao-Wei Wen, · Wlodzimierz Borejsza-Wysocki, Thomas R. DeCory, Antje J. Baeumner Richard A. Durst, “A novel extraction method for peanut allergenic proteins in chocolate and their detection by a liposome-based lateral flow assay” European Food Research and Technology, vol. 221, pp. 564 – 569 (2005)
  • Chien-Sheng Chen, Antje J. Baeumner, Richard A. Durst “Protein G-liposomal nanovesicles as universal reagents for immunoassays” Talanta, vol. 67, pp. 205 – 211 (2005)
  • Antje Baeumner “Bioanalytical Microsystems: Technology and Applications” invited book chapter, chapter 6, pp. 251-284 “Biosensors and Modern Biospecific Analytical Techniques”, (Ed. L. Gorton), Vol. XLIV, Comprehensive Analytical Chemistry (Ser. Ed. D. Barcelo, Elsevier, Amsterdam, 2005. doi:10.1016/S0166-526X(05)44006-4
  • Antje J. Baeumner, Barbara Leonard, John McElwee, Richard A. Montagna “A Rapid Biosensor for Viable B. anthracis Spores” Analytical Bioanalytical Chemistry, vol. 380 (1), p. 15 – 23 (2004)
  • Natalya Zaytseva, Richard A. Montagna, Eun Mi Lee and Antje J. Baeumner “Multi-Analyte Single-Membrane Biosensor for Serotype Specific Detection of Dengue Virus” Analytical Bioanalytical Chemistry, vol. 380 (1), p. 46 – 53 (2004)
  • Antje J. Baeumner, Caroline Jones, Ching Yee Wong, and Andrew Price “A Generic Sandwich-type Biosensor with Nanomolar Detection Limits” Analytical and Bioanalytical Chemistry, vol. 378 (6), pp. 1587 – 1593 (2004)
  • A.J. Baeumner, J. Pretz, S. Fang “A Universal Nucleic Acid Sequence Biosensor with Nanomolar Detection Limits”, Analytical Chemistry, vol. 76 (4), pp. 888 – 894 (2004)
  • Antje Baeumner “Bioanalytical Microsystems: Technology and Applications” invited book chapter, Editor Lo Gorton (Elsevier) accepted in Oct. 2002
  • A.J. Baeumner “Biosensors for Environmental Pollutants and Food Contaminants” Analytical and Bioanalytical Chemistry, vol 377 (3), pp. 434 – 445, (2003) (invited review)
  • J.-H. Min and A.J. Baeumner “Characterization and Optimization of Interdigitated Ultramicroelectrode Arrays as Electrochemical Biosensor Transducers” Electroanalysis, May 2003 (published online, Feb. 20th, 2004)
  • S. Kwakye and A. Baeumner “A Microfluidic Biosensor Based on Nucleic Acid Sequence Recognition” Analytical and Bioanalytical Chemistry, vol. 376 (7), pp. 1062 – 1068 (2003)
  • A.J. Baeumner and R.A. Durst “Foreword: 5th Workshop on Biosensors and Biological Techniques in Environmental Analysis” Analytica Chimica Acta, vol. 487 (1), p. 1, 2003
  • H.A. Hartley and A.J. Baeumner “Biosensor for the Specific Detection of a Single Viable B. anthracis Spore” Analytical and Bioanalytical Chemistry, vol. 376 (3), pp. 319 – 327 (2003) (article published in category “Paper in Forefront”)
  • S. Ahn-Yoon, R.R. DeCory, A.J. Baeumner and R.A. Durst “Ganglioside-Liposome Immunoassay for the Ultrasensitive Detection of Cholera Toxin”, Analytical Chemistry, vol. 75, pp. 2256 – 2261 (2003)
  • J.-H. Min and A.J. Baeumner, “The micro-Total Analytical System for the Detection of Bacteria/Viruses” J. Industrial Engineering Chemistry, vol. 9 (1), pp. 1 – 8 (2003)
  • A.J. Baeumner, R.N. Cohen, V. Miksic, J.H. Min “RNA Biosensor for the Rapid Detection of Viable Escherichia coli in Drinking Water” Biosensors & Bioelectronics, vol. 8 (4) pp. 405 – 419 (2003)
  • M.D. Dhawan, F. Wise, A.J. Baeumner “Development of a Laser-Induced Cell Lysis System” Analytical and Bioanalytical Chemistry, vol. 374, pp. 421 – 426 (2002) 
  • A.J. Baeumner, N. A. Schlesinger, N. S. Slutzki, J. Romano, E.M. Lee, R.A. Montagna “A biosensor for Dengue Virus Detection: Sensitive, Rapid and Serotype specific” Analytical Chemistry, vol. 74 (6), p. 1442 – 1448 (2002)
  • J.-H. Min and A.J. Baeumner “Highly Sensitive and Specific Detection of Viable Escherichia coli in Drinking water” Analytical Biochemistry, vol. 303, p. 186 – 193 (2002)
  • M.B. Esch, A.J. Baeumner, R.A. Durst “Detection of Cryptosporidium parvum Using Oligonucleotide-Tagged Liposomes in a Competitive Assay Format “Analytical Chemistry vol. 73 (13), pp. 3162-3167 (2001)
  • A.J. Baeumner, M.C. Humiston, R.A. Montagna, R.A. Durst “Detection of Viable Oocysts of Cryptosporidium parvum Following Nucleic Acid Sequence-Based Amplification” Analytical Chemistry vol. 73 (6), pp. 1176-1180 (2001)
  • L. Mosiello, L. Segre, S. Chiavarini, C. Cremisini, M. Spano, A.J. Bäumner, T. Kimmel, R.D. Schmid “Dipstick Immunoassay Format for Atrazine and Terbutylazine Analysis in Water Samples.” J. Agricultural and Food Chemistry vol. 46 (9), 3847 - 3851, 1998
  • A.J. Bäumner and R.D. Schmid, “Development of a New Immunosensor for Pesticide Detection: a Disposable System with Liposome-Enhancement and Amperometric Detection” Biosensors & Bioelectronics vol. 13 (5), 519 - 529 (1998)
  • R.A. Durst, A.J. Bäumner, R.W. Murray, R.P. Buck, C.P. Andrieux, “Chemically Modified Electrodes: Recommended Terminology and Definitions” Pure & Applied Chemistry vol. 69 (6), pp. 1317 - 1323 (1997)
  • A.J. Bäumner, T. Kummer, R.D. Schmid, “Liposome-Based Immunosensors: 1. Influence of Hapten Spacer Length on Liposome Binding Efficiency.” Analytical Letters vol 29 (15), pp. 2601-2613 (1996)

speaker at Cornell event

Keywords: AIDS diagnostics, biological engineering, biosensor, biosensors & biocontrol engr, DNA and RNA detection, microfluidic, nanofabrication, nanosensor, pathogen detection