Long, Qiaoming

member of graduate field

other Cornell affiliations

Stem Cell Program (SCP)

research overview

The research in my laboratory is concerned with development, physiology and diseases of the pancreas. Our specific interests include: (1) the molecular pathways directing the specification and differentiation of endocrine pancreatic cells; (2) endocrine regulation of animal growth and body metabolism; and (3) the molecular mechanisms underlying pancreatic beta cell death in diabetes and pancreatic cancers. We are actively working on two research projects. The first is functional characterization of regulatory factor X (RFX) domain-containing 1 (RFXDC1). Rfxdc1 is a novel gene whose transcript and protein are restricted to the islets of Langerhans of the pancreas. Knockdown of RFXDC1 function during zebrafish embryonic development by morpholino antisense oligos causes abnormal pancreatic formation. We hypothesized that RFXDC1 may function as a transcriptional regulator or coregulator during development of the pancreas. We are now trying to generate a knockout mouse model to study the physiological function of Rfxdc1. The second project is investigating the molecular mechanisms of pancreatic beta cell apoptosis in diabetes. We are particularly interested in understanding the causal relationship between endoplasmic reticulum (ER) stress and beta cell death and survival. SEL1L is an ER membrane protein and is highly expressed in the pancreas. It has been shown by other investigators that SEL1L forms a highly conserved protein quality control complex with other proteins on the ER membrane to facilitate degradation of misfolded or unassembled proteins in the ER, which otherwise will aggravate and become cytotoxic. We have generated Sel1L-deficient mouse embryos and are learning to understand the in vivo functions of SEL1L by characterizing the phenotypes of these embryos.

area(s) of concentration/expertise

Genetic regulation

keywords

Notch
diabetes
gene
pancreas

submitted impact statement

individual publications

academic article
- Haploid insufficiency of suppressor enhancer Lin12 1 like (SEL1L) predisposes mice to high fat diet-induced hyperglycemia. Journal of Biological Chemistry. 286:22275-22282. 2011
- Haploid insufficiency of suppressor enhancer Lin12 1-like (SEL1L) protein predisposes mice to high fat diet-inducedhyperglycemia. Journal of Biological Chemistry. 286:22275-82. 2011
- Deficiency of SEL1L in mice leads to systemic ER stress and embryonic lethality. Journal of Biological Chemistry. 285. 2010
- Replacement of Rbpj with Rbpjl in the PTF1 complex controls the final maturation of pancreatic acinar cells . Gastroenterology. 139:270-280. 2010
- SEL1L deficiency impairs growth and differentiation of pancreatic epithelial cells . BMC Developmental Biology. 10:13. 2010
- SEL1L deficiency impairs growth and differentiation of pancreatic epithelial cells . BMC Developmental Biology. 10:13. 2010
- The full-length isoform of the mouse pleckstrin homology domain-interacting protein (PHIP) is required for postnatal growth. FEBS Letters. 584:4121-4127. 2010
- Pdx-1 and Pft1a concurrently determine fate specification of pancreatic multipotent progenitor cells. Developmental Biology. 316:74-86. 2008
- Early pancreatic development requires the vertebrate Suppressor of Hairless (RBPJ) in the PTF1 bHLH complex. Genes and Development. 21:2629-2643. 2007
- Isolation and expression of zebrafish zinc-finger transcription factor gene tsh1. Gene Expressions Patterns. 7:318-322. 2007
- Ptf1a determines horizontal and amacrine cell fates during mouse retinal development. Development. 133:4439-50. 2006
- Efficient DNA cassette exchange in mouse ES cells by staggered positive-negative selection. Genesis. 39:256-262. 2004
- Regulatory roles of conserved intergenic domains in vertebrate Dlx bigene clusters. Genome Research. 13:533-543. 2003
- The ERV-9 LTR enhancer is not blocked by the HS5 insulator and synthesizes through the HS5 site non-coding, long RNAs that regulate LTR enhancer function. Nucleic Acids Research. 31:4582-4596. 2003
- Expression and Regulation of mouse mtsh1 during limb and branchial arch development. Developmental Dynamics. 222:308-312. 2001
- A highly conserved enhancer in the Dlx5/Dlx6 intergenic region is the site of cross-regulatory interactions between Dlx genes in the embryonic forebrain. Journal of Neuroscience. 20:709-721. 2000
- Scyba encodes a Novel CXC-type chemokine and is distinctively expressed in the vestibulo-acoustic system during zebrafish embryogenesis. Mechanisms of Development. 97:195-198. 2000
- Stimulation of erythropoiesis by inhibiting a new hematopoietic death receptor in transgenic zebrafish. Nature Cell Biology. 2:549-552. 2000
- A long terminal repeat of the human endogenous retrovirus ERV-9 is located in the 5'boundary area of the human beta-globin locus control region. Genomics. 54:542-555. 1998
- A zebrafish model for hepatoerythropoietic porphyria. Nature Genetics. 20:239-243. 1998
- Positive and negative cis-acting elements are required for hematopoietic expression of zebrafish GATA-1. Blood. 93:500-508. 1998
- GATA-1 expression pattern can be recapitulated in living transgenic zebrafish using GFP reporter gene. Development. 124:4105-4111. 1997
conference paper
- Dlx regulation in the developing forebrain: Interactions between cis-acting regulatory elements in transgenic mice and zebrafish. Society of Developmental Biology Annual Meeting. 99. 2003
document part
- Cover Crop Practices and Usage Among Dairy Farmers in Washington County, New York 2010

speaker at Cornell event

Mechanisms of Pancreatic Beta Cell Dysfunction and Apoptosis in Type 2 Diabetes

teaching overview

My goal is to develop an advanced growth biology course to translate the basic information gathered from model organisms to explain the mechanisms regulating tissue and whole body growth in species of interest. By working with Dr. Yves Bosclair, I have developed and taught AnSc 3920 (Mechanisms of Animal Growth and Development). The course employs model systems (cell culture, fish and mice) to examine cellular and molecular mechanisms of animal growth and devlopment. Lectures cover: (1) patterns of whole animal growth during fetal and postnatal life; (2) molecular and cellular basis of formation and development of skeletal muscle, adipose tissue and bone; (3) regulation of growth and development by hormones and growth factors; (4) emerging molecular technologies and whole-genome approaches for improving growth and meat quality with discussions about major-effect genes as well as quantitative trait loci.

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CALS

research keyword

Notch
diabetes
gene
pancreas

name prefix

Dr.

VIVO: Cornell Research & Scholarship

Long, Qiaoming

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