Networks
Beer, Steven Vincent
Cornell Faculty Member
Positions
- Professor, Plant Pathology and Plant-Microbe Biology (PPPMB), College of Agriculture and Life Sciences (CALS)
I have expertise in plant pathology, particularly diseases of plants caused by bacteria, especially fire blight of apple, pear and related plants and bacterial diseases of onions. I have functioned broadly in these respects, in teaching, reseach and extension. My research activity has covered epidemiology and control, molecular genetics, genomics and proteomics. Much of my recent activity has been fundamental in nature, however, application of fundamental knowledge to applied problems is a strong motivation. I advise others informaly concerning bacterial diseases and the identification and manipulation of the pathogens.
Research Areas
- agricultural biotechnology
- biochemistry
- bioinformatics
- biological control
- biotechnology
- disease control
- entrepreneurship
- fruit and vegetable production
- genomics
- integrated crop management
- microbiology
- molecular biology
- molecular genetics
- new life sciences
- pathogens
- pest management
- plant biology
- plant pathology
- plant sciences
- proteomics
- science education
- teacher education
- transgenic plants
Websites
- Affiliations
- Research
- Publications
- Teaching
- Service
- Background
- Other
- View All
Affiliations
Research
research overview
- PROFESSIONAL OVERVIEW My responsibility for work on diseases of tree fruit evolved to emphasize molecular genetic studies of phytopathogenic bacteria, particularly the fire blight pathogen, Erwinia amylovora. Concurrently, the Department’s needs for instruction in phytobacteriology led me to offer courses in bacterial plant pathogens for some 15 years. The more recent call for undergraduate courses led me to volunteer to teach a “Senior Seminar” for students majoring in Biology and Society. Research studies over the last twenty-five years resulted in the identification and characterization of many hrp genes that encode products critical to disease development as well as several plant proteins with which the bacterial proteins interact. One of those genes, hrpN encodes a protein (harpin) that has been commercialized as a resistance inducer and plant growth enhancer. Recent research involved characterizing the genome of E. amylovora and determining the possible role of several genes and gene products in interactions with host and non-host plants. Utilization of bacterial genes and their interacting proteins of plants has been explored as an approach to control of plant disease. In addition, the genetic basis of host-specificity of strains of the fire blight pathogen for plants of two subfamilies of the Rosaceae family of plants was explored. In 2007, my program began to address bacterial disease problems of onions. This partial redirection of effort was in response to increasing problems experienced by New York onion growers. A pathogen new to New York onions, Pantoea ananatis, was identified and characterized. A year later, Enterobacter cloacae was newly identified in New York as the cause of serious losses for several large growers of onions. Several other bacteria of concern to New York onion growers are currently being studied. Present emphasis is placed on rapidly identifying and characterizing the bacteria that are responsible for losses, determining their epidemiology, genetics of pathogenicity and developing strategies for effective measures to reduce disease incidence and losses.
research activities
principal investigator on
co-principal investigator on
area(s) of concentration/expertise
other area(s) of concentration/expertise
- Plant Pathology, Microbiology
keywords
- bacteriology
- disease etiology
- fire-blight
- fire-blight, erwinia, disease, bacteria, apple, pear,
- gene-expression
- gene-sequencing
- gene-silencing
- genomics
- host-specificity
- microbiology
- molecular-biology
- molecular-genetics
- onion decay
- phytobacteriology
- phytopathology
- plant-disease
- plant-microbe-biology
- plant-molecular-biology
- transgenic-plants
submitted impact statement
- Understanding the mechanism by which the Fire Blight pathogen causes disease: the key to manipulating apples to make them more resistant to Fire Blight for the benefit of apple producers, consumers, and the general public
- Outreach education for high school students regarding bacterial plant interaction involving genes
- Genome Sequencing of Erwinia amylovora
- Bacterial pathogens of plants, their genes, and the relevance of genome sequencing for secondary school students and teachers.
Publications
individual publications
-
academic article
- Comparative Genomics of 12 Strains of Erwinia amylovora Identifies a Pan-Genome with a Large Conserved Core. PLoS One. 8. 2013
- 'Top 10 Plant Bacteria in Molecular Plant Pathology'. . Molecular Plant Pathology. 13:614-629. 2012
- Comparative analysis of the Hrp pathogenicity island of Rubus- and Spiraeoideae-infecting Erwinia amylovora strains identifies the IT region as a remnant of an integrative conjugative element. Gene. 504:6-12. 2012
- Exploring the relationship between nitrogen, plant spacing and bacterial diseases of onion in New York: reduced nitrogen and closer spacing could result in less rot.. Onion World. 28:18-21. 2012
- HopX1 in Erwinia amylovora functions as an avirulence protein in apple and is regulated by HrpL.. Journal of Bacteriology. 194:553-560. 2012
- OEM--a new medium for rapid isolation of onion-pathogenic and onion-associated bacteria.. Journal of Microbiological Methods. 91:520-526. 2012
- An analysis of the Erwinia amylovora pan-genome identifies novel chromosomal targets for molecular diagnostics. . Acta Horticulturae. 896:45-50. 2011
- Eop1 from a Rubus strain of Erwinia amylovora functions as a host-range limiting factor. . Phytopathology. 101:935-944. 2011
- First Report of Enterobacter Bulb Decay of Onions Caused by Enterobacter cloacae in New York.. Plant Disease. 95:1581. 2011
- Genome sequence of an Erwinia amylovora strain with restricted pathogenicity to Rubus plants.. Journal of Bacteriology. 193:785-786. 2011
- The specificity of PCR-based protocols for detection of Erwinia amylovora. . Australasian Plant Pathology. 40:87-97. 2011
- Complete genome sequence of the plant pathogen Erwinia amylovora strain ATCC 49946.. Journal of Bacteriology. 192:2. 2010
- First report of a bulb disease caused by Pantoea ananatis in New York. . Plant Disease. 94:916. 2010
- Secretion and translocation signals and DspB/F-binding domains in the type III effector DspA/E of Erwinia amylovora.. Microbiology. 156:1211-1220. 2010
- Genetic transformation of Citrus sinensis cv. Hamlin with hrpN gene from Erwinia amylovora and evaluation of the transgenic lines for resistance to citrus canker.. Scientia Horticulturae. 122:109-115. 2009
- A fragment of the Xanthomonas oryzae pv. oryzicola harpin HpaGxooc reduces disease and increases yield of rice in extensive grower plantings. Phytopathology. 98:792-802. 2008
- HrpN of Erwinia amylovora functions in the translocation of DspA/E into plant cells.. Molecular Plant Pathology. 9:425-434. 2008
- Identification of specific fragments of HpaGxooc, a harpin from Xanthomonas oryzae pv. oryzicola, that induce disease resistance and enhance growth in plants. Phytopathology. 98:781-791. 2008
- Some cellular correlates of harpin-induced resistance to blue mold of apples. Tropical Plant Pathology. 33:103-113. 2008
- The C-terminal half of the HrpN virulence protein of the fire blight pathogen Erwinia amylovora is essential for its secretion and for its virulence and avirulence activities. Molecular Plant Microbe Interactions. 21:1387-1397. 2008
- Analyses of the secretomes of Erwinia amylovora and selected hrp mutants reveal novel type III secreted proteins and an effect of HrpJ on extracellular harpin levels. Molecular Plant Pathology. 8:55-67. 2007
- AtHIPM, an ortholog of the apple HrpN-interacting protein, is a negative regulator of plant growth and mediates the growth-enhancing effect of HrpN in Arabidopsis. Plant Physiology. 145:426-436. 2007
- DspA/E, a type III effector of Erwinia amylovora, is required for early rapid growth in Nicotiana benthamiana and causes NbSGT1-dependent cell death. Molecular Plant Pathology. 8:255-265. 2007
- Expression of phi-Ea1h phage depolymerase gene with constitutive and inducible promoters, translation enhancer, and signal sequence in transgenic apple plants increases resistance to fire blight. Acta Horticulturae. 738:273-276. 2007
- Strategies for obtaining fire blight resistance in apple by rDNA technology. Acta Horticulturae. 738:283-285. 2007
- Apple proteins that interact with DspA/E, a pathogenicity effector of Erwinia amylovora, the fire blight pathogen. Molecular Plant-Microbe Interactions. 19:53-61. 2006
- Application of signature tagged mutagenesis to the study of virulence of Erwinia amylovora. FEMS Microbiology Letters. 265:164-171. 2006
- PR genes of apple: identification and expression in response to elicitors and inoculation with Erwinia amylovora. BMC Plant Biology. 6:23. 2006
- The Hrp pathogenicity island of Erwinia amylovora and identification of three novel genes required for systemic infection. Molecular Plant Pathology. 6:125-138. 2005
- Downstream divergence of the ethylene signaling pathway for harpin-stimulated Arabidopsis growth and insect defense. Plant Physiology. 136:3628-3638. 2004
- The Erwinia chrysanthemi EC16 hrp/hrc gene cluster encodes an active Hrp type III secretion system that is flanked by virulence genes functionally unrelated to the Hrp system. Molecular Plant-Microbe Interactions. 17:644-653. 2004
- Development of Fire Blight Resistant Apple Cultivars by Genetic Engineering. Acta Horticulturae. 622:105-111. 2003
- Harpin-elicited hypersensitive cell death and pathogen resistance requires the NDR1 and EDS1 genes. Physiological and Molecular Plant Pathology. 62:317-326. 2003
- Pre- and post-harvest harpin treatments of apples induce resistance to blue mold. Plant Dis.. 89:39-44. 2003
- Structural and functional analysis of Pantocin A: An antibiotic from Pantoea agglomerans discovered by heterologous expression of cloned genes. Angew. Chem. Int. Ed. 42:2898-2901. 2003
- The biosynthetic gene cluster of Pantocin A provides insights into biosynthesis and a tool for screening. Angew. Chem. Int. Ed.. 42:2902-2905. 2003
- Alternative disease control agents induce resistance to blue mold in harvested ‘Red Delicious’ apple fruit. Phytopathology. 92:900-908. 2002
- Pantoea agglomerans strain EH318 produces two antibiotics that inhibit Erwinia amylovora in vitro. Applied and Environmental Microbiology. 67:284-292. 2001
- Regulation of hrp genes and type III protein secretion in Erwinia amylovora by HrpX/HrpY, a novel two-component system, and HrpS. Mol. Plant-Microbe Interact.. 11:1251-1262. 2000
- Riboflavin induces disease resistance in plants by activating a novel signal transduction pathway. Phytopathology. 90:801-811. 2000
- Assessment of phenotypic variability in Erwinia stewartii based on metabolic profiles. Plant Disease. 83:114-118. 1999
- Harpin induces disease resistance in Arabidopsis through the systemic acquired resistance pathway mediated by salicylic acid and NIM1 gene. Plant J.. 20:207-215. 1999
- Pantocin B, an antibiotic from Erwinia herbicola discovered by heterologous expression of cloned genes. Journal of the American Chemical Society. 121:11912-11913. 1999
- Erwinia amylovora secrets DspE, a pathogenicity factor and functional AvrE homolog through the Hrp (Type III Secretion) pathway. Journal of Bacteriology. 180:2244-2247. 1998
- Homology and functional similarity of an hrp-linked pathogenicity locus, dspEF, of Erwinia amylovora and the avirulence locus avrE of Pseudomonas syringae pathovar tomato. Proceedings at the National Academy of Science USA. 95:1325-1330. 1998
- HrpW of Erwinia amylovora, a new harpin that contains a domain homologous to pectate lyases of a distinct class. Journal of Bacteriology. 180:5203-5210. 1998
- Sequences related to transposable elements and bacteriophages flank avirulence genes of Pseudomonas syringae. Mol. Plant-Microbe Interact.. 11:1247-1252. 1998
- The hrpC and hrpN operons of Erwinia chrysanthemi EC16 are flanked by plcA and homologs of hemolysin/adhesin genes and accompanying activator/transporter genes.. Mol. Plant-Microbe Interact.. 11:563-567. 1998
- Characterization of Erwinia amylovora strains using random amplified polymorphic DNA fragments (RAPDs). Journal of Applied Microbiology. 82:389-398. 1997
- The hrpA and hrpC operons of Erwinia amylovora encode components of a type III pathway that secretes harpin. Journal of Bacteriology. 179:1690-1697. 1997
- Antagonism of selected bacterial strains to Stemphylium vesicarium and biological control of brown spot of pear under controlled environment conditions. Phytopathology. 86:856-863. 1996
- Characterization of bacteria that cause "bacterial shoot blight of pear" in Japan. Acta Horticulturae. 411:179-181. 1996
- Erwinia amylovora secretes harpin via a Type III pathway and contains a homolog of yopN of Yersinia spp. Journal of Bacteriology. 178:1720-1730. 1996
- Unified nomenclature for broadly conserved hrp genes of phytopathogenic bacteria. Molecular Microbiology. 20:681-683. 1996
- Erwinia chrysanthemi harpinEch: An elicitor of the hypersensitive response that contributes to soft-rot pathogenesis. Mol. Plant-Microbe Interact.. 8:484-491. 1995
- hrpL activates Erwinia amylovora hrp gene transcription and is a member of the ECF subfamily of d factors. Journal of Bacteriology. 177:6201-6210. 1995
- Erwinia chrysanthemi hrp genes and their involvement in soft rot pathogenesis and elicitation of the hypersensitive response. Molecular Plant-Microbe Interactions. 5:573-581. 1994
- Mechanisms of inhibition of Erwinia amylovora by E. herbicola in vitro and in vivo. J. Appl. Bacteriol.. 76:30-35. 1994
- The hypersensitive response elicited by Erwinia amylovora harpin requires active plant metabolism. Molecular Plant-Microbe Interactions. 7:289-292. 1994
- HrpI of Erwinia amylovora functions in secretion of harpin and is a member of a new protein family. Journal of Bacteriology. 175:7958-7967. 1993
- Expression of Erwinia amylovora hrp genes in response to environmental stimulii. Journal of Bacteriology. 174:1875-1882. 1992
- Harpin, elicitor of the hypersensitive response produced by the plant pathogen Erwinia amylovora. Science. 257:85-88. 1992
- Hybridization and functional complementation of the hrp gene cluster from Erwinia amylovora strain Ea321 with DNA of other bacteria. Molecular Plant-Microbe Interactions. 5:412-419. 1992
- Further characterization of an hrp gene cluster of Erwinia amylovora. Molecular Plant-Microbe Interactions. 4:493-499. 1991
- Role of antibiotic production by Erwinia herbicola Eh252 in biological control of Erwinia amylovora. Journal of Bacteriology. 174:2785-2796. 1991
- Characterization of a 56-kb plasmid of Erwinia amylovora Ea322: Its noninvolvement in pathogenicity. Plasmid. 24:12-24. 1990
- Control of fire blight by Erwinia herbicola Eh252: Role of antibiotic production. Acta Horticulturae. 273:393-394. 1990
- Creation and complementation of pathogenicity mutants of Erwinia amylovora. Molecular Plant-Microbe Interactions. 1:135-144. 1988
- Virulence of Erwinia amylovora strains to Malus sp. Novole plants grown in vitro and in the greenhouse. Phytopathology. 78:1292-1297. 1988
- An assay for the virulence of Erwinia amylovora using Malus tissue culture. Acta Horticulturae. 217:283. 1987
- Evaluation and selection of fire blight resistance in apple with reference to differential virulence in Erwinia amylovora. Acta Horticulturae. 217:263-264. 1987
- Interactions between actinomycete-like organisms and young apple roots grown in soil conducive to apple replant disease. Phytopathology. 77:1071-1077. 1987
- Multiple regression analysis of factors associated with the occurrence of blossom infection in the Northeastern United States. Acta Horticulturae. 217:99. 1987
- The effects of virulence of Erwinia amylovora on the evaluation of fire blight resistance in Malus. Phytopathology. 77:1551-1555. 1987
- Differential susceptibility of Malus spp. cultivars Robusta 5, Novole, and Ottawa-523 to Erwinia amylovora. Plant Dis.. 70:1017-1019. 1986
- Fireblight in Israel-1985. Observations and recommendations. EPPO Bull.. 16:639-646. 1986
- Infection of apple roots by actinomycetes associated with soils conducive to apple replant disease. Plant Dis.. 70:1125-1128. 1986
- Scanning electron microscopy of apple blossoms colonized by Erwinia amylovora and E. herbicola. Phytopathology. 76:900-904. 1986
- Evidence that a putative necrotoxin of Erwinia amylovora is an artifact caused by the activity of inorganic salts. Physiol. Plant Pathol.. 27:289-298. 1985
- Differential host X pathogen interactions among cultivars of apple and strains of Erwinia amylovora. Phytopathology. 74:136-139. 1984
- Differential virulence of Erwinia amylovora to specific apple cultivars and its implications for breeding and selecting fire blight resistant plants. Acta Horticulturae. 151:255-258. 1984
- Towards a Practical Warning System for Fire Blight Blossom Infection. Acta Horticulturae. 151:23-36. 1984
- Amylovorin-induced shoot wilting: Lack of correlation with susceptibility to Erwinia amylovora. Phytopathology. 73:1328-1333. 1983
- An exo-poly-a-D-gal acturonosidase implicated in the regulation of extracellular pectate lyase production in Erwinia chrysanthemi. Journal of Bacteriology. 149:626-634. 1982
- Plasmid borne determinants of pigmentation and thiamine prototrophy in Erwinia herbicola. Journal of Bacteriology. 151:1627-1629. 1982
- Transfer of the drug-resistance transposon Tn5 to Erwinia herbicola and the induction of insertion mutations. Current Microbiology. 6:377-381. 1981
- Hydrogen cyanide sensitivity in bacterial pathogens of cyanogenic and non-cyanogenic plants. Phytopathology. 70:1005-1008. 1980
- Resistance and seed infection in three dry bean cultivars exposed to a halo blight epidemic. Plant Dis.. 4:857-859. 1980
- The potential of four dry bean cultivars to serve as sources of Pseudomonas phaseolicola inoculum. Plant Dis.. 64:72-74. 1980
- Fire blight inoculum: Sources and dissemination. EPPO Bull.. 9:13-25. 1978
- Mechanism of wilt induction by amylovorin in cotoneaster shoots and its relation to wilting of shoots infected by Erwinia amylovora. Phytopathology. 68:89-94. 1978
- Fire blight epidemiology: Factors affecting release of Erwinia amylovora by cankers. Phytopathology. 67:1119-1125. 1977
- Isolation and partial characterization of two neutral proteases of Erwinia amylovora. Phytopath. Zeit.. 90:12-21. 1977
- Absence of cell wall polysaccharide degradation by Erwinia amylovora. Phytopathology. 66:433-436. 1976
- Erwinia amylovora on fire blight canker surfaces and blossoms in relation to disease occurrence. Phytopathology. 66:317-322. 1976
- Fire blight control with streptomycin sprays and adjuvants at different application volumes. Plant Dis. Reptr.. 60:541-544. 1976
- Fire blight in the Geneva apple collection. Fruit Var. J.. 30:42-55. 1976
- Nutrient status of apple blossoms and their susceptibility to fire blight. Annals of Applied Biology. 81:159-163. 1976
- Streptomycin-resistant Erwinia amylovora not found in western New York pear and apple orchards. Plant Dis. Reptr.. 60:624-626. 1976
- Spermidine and spermine-polyamine components of turnip yellow mosaic virus. Virology. 40:930-938. 1970
- Simultaneous production and synergistic action of oxalic acid and polygalacturonase during pathogenesis by Sclerotium rolfsii. Phytopathology. 55:204-211. 1965
-
article
- Better understanding of bacterial onion diseases in New York. Onion World. 28:18-23. 2012
- Exploring the relationship between nitrogen, plant spacing and bacterial diseases in New York. Onion World. 28:18-21. 2012
- Understanding bacterial diseases of onion in New York 2012
- Studies of bacterial problems of onion in New York _ 2010 2011
- Nitrogen, phosphorus, and potassium removal by brown midrib sorghum sudangrass 2006
- Nitrogen management for brown midrib sorghum sudangrass: Results of six NY field studies in 2004 2005
- Nitrogen management for brown mid rib sorghum sudangrass. Results of two years of studies at the Mt Pleasant Research Farm 2004
- Potassium management for brown mid rib sorghum sudangrass. Results of two years of studies at the Mt Pleasant Research Farm 2004
- Bacterial Plant Pathology: Cell and Molecular Aspects. (a review) 1994
- Plant Diseases: Their Biology and Social Impact (a review) 1992
- Fighting fire blight. Am. Fruit Grower. 97. 1977
- Fire blight in New York State 1974
- Fire blight in New York State 1974
-
booksection
- Disease-specific genes of Erwinia amylovora: Keys to understanding pathogenesis and potential targets for disease control 2000
- hrp Genes and Harpins of Erwinia amylovora: A Decade of Discovery 2000
- hrp Genes and their function 1998
- Graduate Study in Plant Pathology 1993
- Graduate Study in Plant Pathology 1992
- Graduate Study in Plant Pathology 1991
- Biological control of Erwinia amylovora 1990
- Fire Blight 1990
- Evaluation of spray materials to control fire blight: Laboratory, greenhouse and field techniques 1986
- Apple 1982
- Fire blight 1982
- Fire blight and its control. Vol. Vol 1. 1979
- Techniques for field evaluation of spray materials to control fire blight of apple and pear blossoms 1978
- The dearth of and needs for new bactericides. Vol. 31. 1976
-
chapter
- Apple. McGraw-Hill Encyclopedia of Science and Technology, 5th Edition. 642-645. 1982
- Fire blight and its control. Horticulture Reviews. 425-476. 1979
-
conference paper
- Research yields greater understanding of bacterial diseases of onion in New York. 2012 Empire State Fruit & Vegetable Expo Proceedings. http://www.hort.cornell.edu/expo/2012proceedings.php. 2012 Empire State Fruit & Vegetable Expo Proceedings.. 6. 2012
- Research yields greater understanding of bacterial diseases of onion in New York. 2012 Empire State Fruit & Vegetable Expo Proceedings. http://www.hort.cornell.edu/expo/2012proceedings.php. 2012 Empire State Fruit & Vegetable Expo Proceedings.. 6. 2012
- Studies of bacterial problems of onion in New York -- 2010. Proceedings of the 2011 Empire State Fruit & Vegetable Expo.. 105-107. 2011
- Recent studies of bacterial problems of onion. Cornell University Coopertive Extension. 105-107. 2010
- Recent studies of bacterial problems of onions in New York. Proceedings of The 2010 Empire State Fruit and Vegetable Expo. 105-107. 2010
- Pathogenesis of Pantoea ananatis causing center rot of onions . Proceedings of The 2009 Empire State Fruit and Vegetable Expo. 50-53. 2009
- Contributions of the genome sequence of Erwinia amylovora to the fire blight community. Proceedings of the Eleventh International Workshop on Fire blight. 163-170. 2008
- Detection of Pantoea ananatis in New York Grown Onions and Devleopment of Pathogenicity Tests for P. ananatis on Onion. Proceedings of the 2008 National Allium Research Conference. 2008
- Evaluation of PCR-based protocols for the detection of Erwinia amylovora. Proceedings of the Eleventh International Workshop on Fire blight. 505-511. 2008
- Increased resistance to fire blight by expression of the phi-ea1h phage depolymerase gene with constitutive and inducible promoters, translation enhancer and signal sequence in transgenic apple. Proceedings of the Eleventh International Workshop on Fire blight. 293-296. 2008
- Increased resistance to fire blight by expression of the phi-ea1h phage depolymerase gene with constitutive and inducible promoters, translation enhancer and signal sequence in transgenic apple. Acta Horticulturae. 293-296. 2008
- Occurrence in New York of Pantoea ananatis, the cause of center rot of onion. Proceedings of 2008 Empire State Fruit and Vegetable Expo and Becker Forum. 2008
- eop1 differs in strains of Erwinia amylovora that differ in host specificity. Proceedings of the Eleventh International Workshop on Fire blight. 213-214. 2008
- DspE, an effector of Erwinia amylovora, is translocated into plant cells. Proceedings X International Workshop on Fire Blight. 467-471. 2006
- Interaction of DspE/A, a pathogenicity/avirulence protein of Erwinia amylovora, with pre-ferredoxin from apple and its relationship to photosynthetic efficiency. Proceedings X International Workshop on Fire Blight. 473-477. 2006
- Sequencing the entire genome of Erwinia amylovora. Proceedings X International Workshop on Fire Blight. 395-396. 2006
- The biosynthetic genes of pantocin A and pantocin B of P. agglomerans Eh318. Proceedings X International Workshop on Fire Blight. 313-319. 2006
- The fire blight resistance of apple clones in which DspE interacting proteins are silenced. Acta Horticulturae. 509-513. 2006
- The secretion of EopB from Erwinia amylovora. Proceedings X International Workshop on Fire Blight. 409-415. 2006
- Pantoea agglomerans, a biocontrol agent and ubiquitous microorganism - friend or foe?. Proceedings of the 1st Symposium of Biocontrol of Bacterial Plant Diseases. Darmstadt, Germany. 334-338. 2005
- Characterization of left end of the pathogenicity island of Erwinia amylovora. Phytopathology. 2002
- DspE of Erwinia amylovora interacts with receptor kinases of apple. Acta Horticulturae. 469-475. 2002
- Gene expression in apple in response to inoculation with Erwinia amylovora. Acta Horticulturae. 463-466. 2002
- HR induction in tobacco by Erwinia amylovora requires DspE in addition to a harpin. Phytopathology. 2002
- Possible role of a putative nuclear localization signal (NLS) In dspE, a Pathogenicity/Avirulence gene of Erwinia amylovora. Phytopathology. 2002
- Weaponry revealed: secreted proteins of Erwinia amylovora. Phytopathology. 2002
- Effect of expression of hrpN in apple on resistance to Erwinia amylovora. Vitro Cellular and Developmental Biology: 2000 World Congress on In Vitro Biology. 2000
- Analysis of three bacterial strains isolated from symptomatic plants from Australia. Acta Hortic.. 149-157. 1999
- Evaluation of the hrpN gene for resistance to fire blight in apple. Acta Hortic.. 247-250. 1999
- Genetic transformation for fire blight resistance in apple. Proc. 8th International Workshop on Fire Blight. October, 1998. 1999
- Genetic transformation for fire blight resistance in apple.. Acta Hortic.. 295-296. 1999
- New approaches to the development of transgenic plants resistant to fire blight. Acta Hortic.. 301-304. 1999
- Relatedness of Erwinia amylovora strains based on amplied 16S-23S ribosomal DNA restriction enzyme analysis-ardrea. Acta Hortic.. 55-59. 1999
- Secreted enigmatic proteins of Erwinia amylovora—for good and evil. Acta Hortic.. 371-375. 1999
- The hrp pilus and extracellular proteins of Erwinia amylovora. Acta Hortic.. 315-319. 1999
- Analyses of three bacterial strains isolated from symptomatic plants from Australia. Proc. 8th International Workshop on Fire Blight. October, 1998. 1998
- Analysis of three bacterial strains isolated from symptomatic plants from Australia. Proc. 8th Inter. Workshop on Fire Blight. 1998
- Evaluation of the hrpN gene for resistance to fire blight in apple. Proc. 8th Inter. Workshop on Fire Blight. 1998
- Evaluation of the hrpN gene for resistance to fire blight in apple. Proc. 8th International Workshop on Fire Blight. October, 1998. 1998
- Genetic transformation for fire blight resistance in apple. Proc. 8th Inter. Workshop on Fire Blight. 1998
- New approaches to the development of transgenic plants resistant to fire blight. Proc. 8th Inter. Workshop on Fire Blight. 1998
- New transgenic approaches for possible increased resistance to fire blight. Proc. 8th International Workshop on Fire Blight. October, 1998. 1998
- Relatedness of Erwinia amylovora strain based on amplied 16S-23S ribosomal DNA restriction enzyme analysis – ARDREA. Proc. 8th International Workshop on Fire Blight. October, 1998. 1998
- Relatedness of Erwinia amylovora strains based on amplied 16S-23S ribosomal DNA restriction enzyme analysis-ardrea. Proc. 8th Inter. Workshop on Fire Blight. 1998
- Secreted enigmatic proteins of Erwinia amylovora—for good and evil. Proc. 8th Inter. Workshop on Fire Blight. 1998
- Genetics of pantocin a and pantocin b production and their role in biocontrol of the fire blight pathogen, Erwinia amylovora. Proc. Inter. Confer. on Biological Control Bull.. 13-17. 1997
- Characterization of Erwinia amylovora strains from different host and geographical areas. Acta Hortic.. 183-185. 1996
- Characterization of bacteria that cause “bacterial shoot blight of pear” in Japan. Acta Hortic.. 179-181. 1996
- Harpin from Erwinia amylovora induces plant resistance. Acta Hortic.. 223-225. 1996
- Identification of an Erwinia amylovora gene encoding catalase. Acta Hortic.. 227-228. 1996
- The hrp gene cluster of Erwinia amylovora causes electrolyte leakage and necrosis in apple leaves and facilitates bacterial multiplication. Acta Hortic.. 219-221. 1996
- The hrp gene cluster of Erwinia amylovora contains homologs of ysc genes of Yersinia. Acta Hortic.. 265-271. 1996
- The role of antibiotics in biological control of fire blight by Erwinia herbicola strain Eh318. Acta Hortic.. 309-311. 1996
- Application of capillary liquid chromatography: Electrospray mass spectrometry to identify major siderophores of Erwinia amylovora as proferrioxamines and their potential role in virulence. Molecular Mechanisms of Bacterial Virulence. 1994
- Harpin, from Erwinia amylovora, elicits the hypersensitive response and is a determinant of pathogenicity. Acta Hortic.. 243-244. 1993
- Are harpins universal elicitors of the hypersensitive response of phytopathogenic bacteria?. Advances in Molecular Genetics of Plant-Microbe Interactions. 1992
- Effect of induced protection of the expression of hrp genes of Erwinia amylovora in tobacco leaves. Proc. Plant Pathogenic Bacteria Symposium. 1992
- Development of hrp– mutants of Pseudomonas syringae pv. aptata by marker-exchange mutagenesis. Proc. 4th Inter. Working Group on Pseudomonas syringae pathovars. 1991
- Identification of Pseudomonas syringae pv. glycinea based on hybridization to a DNA probe. Proc. 4th Inter. Working Group on Pseudomonas syringae Pathovars. 1991
- The hrp gene cluster of Erwinia amylovora. Advances in Molecular Genetics of Plant-Microbe Interactions. Proc. 5th Inter. Symposium on Molecular Genetics of Plant-Microbe Interactions. 1991
- A cluster of pathogenicity genes from Erwinia amylovora enables Escherichia coli to elicit the hypersensitive response. Acta Hortic.. 241. 1990
- Antibiotics produced by strains of Erwinia herbicola that are highly effective in suppressing fire blight. Acta Hortic.. 411-412. 1990
- Control of fire blight by Erwinia amylovora Eh252: Role of antibiotic production. Acta Hortic.. 393-394. 1990
- Elicitation of the hypersensitive response by Escherichia coli containing a cluster of pathogenicity genes from Erwinia amylovora. Proc. 7th Inter. Conf. Plant Pathogenic Bacteria. 1990
- Mechanism by which the antibiotic of Erwinia herbicola Eh318 inhibits Erwinia amylovora Ea273. Proc. 7th Inter. Conf. Plant Pathogenic Bacteria. 1990
- Opening address, 5th International Workshop on Fire Blight. Acta Hortic.. 19-20. 1990
- Population dynamics of nonpathogenic mutants of Erwinia amylovora in apple host tissue. Acta Hortic.. 239-240. 1990
- Role of antibiotic production by Erwinia herbicola Eh252 in reducing the incidence of fire blight. Proc. Seventh International Conf. Plant Pathogenic Bacteria. 1990
- Siderophore genes of Erwinia amylovora: Cloning and putative role in virulence. Acta Hortic.. 255-257. 1990
- Development of a risk-assessment system for fire blight. Proc. VI Intl. Conf. on Plant Pathogenic Bacteria. 1988
- An assay for the virulence of Erwinia amylovora using Malus tissue culture. Acta Hortic.. 283. 1987
- Attempts to find the mechanism by which Erwinia herbicola inhibits Erwinia amylovora. Acta Hortic.. 223-228. 1987
- Cloning and restriction map of the recA-like gene of Erwinia amylovora. Acta Hortic.. 173-174. 1987
- Cloning of Erwinia amylovora DNA responsible for pathogenicity and the induction of the hypersensitive reaction. Acta Hortic.. 169-170. 1987
- Cloning of a gene from Erwinia amylovora involved in induction of hypersensitivity and pathogenicity. Proc. 6th Int. Conf. Plant Path. Bacteria. 1987
- Disinfestants for treating pruning shears used for fire blight infections. Acta Hortic.. 243. 1987
- Evaluation and selection of fire blight resistance in apple with reference to differential virulence in Erwinia amylovora. Acta Hortic.. 263-264. 1987
- Factors affecting production of herbicolacin 112Y. Proc. 6th Inter. Conf. Plant Pathogenic Bacteria. 1987
- Invasion of the epidermis and cortex of apple roots by actinomycetes. Proc. 6th Inter. Conf. Plant Pathogenic Bacteria. 1987
- Multiple regression analysis of factors associated with the occurrence of blossom infection in the northeastern United States. Acta Hortic.. 99. 1987
- Mutants of Erwinia amylovora altered in pathogenicity by transposon mutagenesis. Acta Hortic.. 167-168. 1987
- Orchard evaluation of five strains of Erwinia herbicola for control of blossom infection. Acta Hortic.. 219. 1987
- Population dynamics of Erwinia amylovora and a biological control agent, Erwinia herbicola on apple blossom parts. Acta Hortic.. 221-222. 1987
- Role of amino acids in biological control of Erwinia amylovora by Erwinia herbicola. Proc. 6th Int. Conf. Plant Pathogenic Bacteria. 1987
- Survival of an introduced strain and natural populations of Erwinia herbicola on apple. Acta Hortic.. 245-251. 1987
- The effect of osmotic concentration on the production of herbicolacin 112Y by Erwinia herbicola 112Y. Acta Hortic.. 229-233. 1987
- Development of a risk-assessment system for fire blight. Proc. 6th Int. Conf. Plant Path. Bacteria. 1986
- Development of a risk-assessment system for fire blight. Proc. 6th Inter. Conf. Plant Pathogenic Bacteria. 1985
- Differential virulence of Erwinia amylovora to specific apple cultivars and its implications for breeding and selecting fire blight resistant plants. Acta Hortic.. 255-258. 1984
- Factors affecting the development of fire blight blossom infections. Acta Hortic.. 37-40. 1984
- Interaction between Erwinia amylovora and Erwinia herbicola in vitro, in immature pear fruits and in apple blossoms. Acta Hortic.. 203-204. 1984
- Recent progress in the development of biological controls for fire blight—A review. Acta Hortic.. 195-202. 1984
- Studies on the mechanism of pathogenesis of Erwinia amylovora. Acta Hortic.. 233-234. 1984
- Towards a practical warning system for fire blight blossom infection. Acta Hortic.. 23-36. 1984
- Towards a practical warning system for fire blight blossom infection. Acta Hortic. 23-26. 1984
- Biological control of fire blight. Acta Hortic.. 123. 1981
- Biological control of fire blight by Erwinia herbicola. Proc. 5th Inter. Conf. Plant Pathogenic Bacteria. 1981
- Fire blight—An update. Proc. N.Y. State Hort. Soc.. 172-178. 1981
- Transfer of the drug-resistance transposon Tn5 to Erwinia herbicola. Proc. 5th Inter. Conf. Plant Pathogenic Bacteria. 1981
- Differences in response of apple (Malus pumila) cultivars to inoculation with Erwinia amylovora. Proc. 4th Int. Conf. Plant Pathogenic Bacteria. 505-512. 1979
- Differences in response of apple (Malus pumila) cultivars to inoculation with Erwinia amylovora. Proc. 4th Int. Conf. Plant Path. Bacteria. 1978
- Distribution of Erwinia amylovora and amylovorin in apple (Malus pumila) shoots inoculated with the fire-blight pathogen. Proc. 4th Int. Conf. Plant Path. Bacteria. 1978
- Effect of Bordeaux mixture-oil sprays on fire blight cankers. Acta Hortic.. 71-76. 1978
- Can amylovorin be used to screen apple cultivars for resistance to Erwinia amylovora?. Proc. 3rd Workshop on Fire Blight Research. 1976
- Curative effects of tetracycline on apple fire blight. Proc. 3rd Workshop on Fire Blight Research. 1976
- Epidemiology of fire blight blossom infection. Proc. 3rd Workshop on Fire Blight Research. 1976
- Factors affecting the sensitivity of rosaceous shoots to amylovorin-induced wilt. Proc. 3rd Workshop on Fire Blight Research. 1976
- Fire blight cankers—Characteristics of inoculum producers, detection and prevention of activity. Proc. 3rd Workshop on Fire Blight Research. 1976
- Glyodin improves streptomycin control of fire blight blossom infection. Proc. 3rd Workshop on Fire Blight Research. 1976
- Recent research on and current recommendations for fire blight control in New York. Proc. 3rd Workshop on Fire Blight Research. 1976
- Mycotoxins in apple juice. Proc. N.Y. State Hort. Soc.. 165-168. 1974
-
document part
- Transposon mutagenesis of Pantoea ananatis: Isolation and characterization of a Tn5-induced mutant with reduced virulence to onion. 144. 2010
- Increased resistance to fire blight by expression of the Phi-Ea1h phage depolymerase gene with constitutive and inducible promoters, translation enhancer and signal sequence in transgenic apple 2007
- Silencing of an apple gene encoding HIPM protein that interacts with HrpN of Erwinia amylovora increases resistance to fire blight 2007
- Increased resistance to fire blight in apple plants by silencing DspE-interacting proteins 2006
- Increasing Resistance To Fire Blight In Transgenic Apple Plants By Expressing Phi-Ea1h Phage Depolymerase Gene With Constitutive And Inducible Promoters, Translation Enhancer And Signal Sequence 2006
- Expression of phage depolymerase gene with constitutive and inducible promoters, translation enhancer and signal sequence in transgenic apple plants increases resistance to fire blight. 18. 2005
- Increased resistance to fire blight in apple plants by silencing DspE-intercating proteins 2005
- Silencing of DspE interacting proteins increases resistance to fire blight of susceptible apple genotypes 2005
- Strategies for obtaining fire blight resistance in apple by rDNA technology. 19. 2005
- Using rDNA technology to obtain fire blight resistance in apple fruit cultivars 2005
- Silencing of Apple Proteins that Interact with DspE, a Pathogenicity Effector from Erwinia amylovora, increases resistance to fire blight 2004
- Apple proteins that interact with DspE, a pathogenicity effector from Erwinia amylovora 2003
- Silencing of Apple Proteins that Interact with DspE, a Pathogenicity Effector from Erwinia amylovora, as a Strategy to Increase Resistance to Fire Blight 2003
- Differential expression of PR genes in apple in response to SAR inducers and Erwinia amylovora 2001
- Harpin-induced resistance for the control of blue mold of apples 2001
- Resistance of hrpN-transgenic M.26 apple rootstock plants to Erwinia amylovora 2001
- The hrp gene cluster is required for activation of Pgst1 by Erwinia amylovora in transgenic apple 2001
- Harpin for insect control 1998
- Phenotypic variability in Pantoea stewartii based on metabolic profiles 1996
-
report
- Patulin—Problems and prevention 1978
- Control of fire blight blossom infection with streptomycin, Bordeaux mixture and an experimental bactericide, 1976 1977
- Effect of oxytetracycline on fire blight lesion extension, 1976 1977
- Glyodin solution improves streptomycin control of fire blight blossom infection, 1976 1977
- Control of fire blight with fixed copper 1976
- Fire blight—Its nature and control 1976
- Preliminary tests of an experiment compound for the control of fire blight 1976
- Apple-fire blight 1975
- Pear-fire blight 1975
- Apple-fire blight 1974
- Apple-postharvest decay 1974
- Apple-postharvest decay 1973
- Pear-fire blight 1973
- Apple-fire blight 1972
- Pear-fire blight 1972
- Molecular genetics of Erwinia amylovora involved in the development of fire blight 2005
- Pantoea agglomerans as a biocontrol agent of fire blight 2005
- Fire Blight—Its Nature, Prevention and Control. 2nd ed. 1999
- Fire blight—Its nature, prevention, and control 1999
- Fire blight—Its Nature, Prevention and Control 1995
- Fire blight—Its nature, prevention, and control 1995
- Fire blight—Its Nature, Prevention and Control 1992
- Fire blight—Its nature, prevention, and control 1992
Teaching
teaching overview
- Ithaca Coodindinator of PLPA 6820, Graduate Student Research Updates
- The recent call for more undergraduate courses, and particularly Biology and Society "Senior Seminars" led me to volunteer to teach a “Senior Seminar” for students majoring in Biology and Society. That course, "Microbes and Food: Contemporary Issues Affecting Humanity", BIOS 4161 and PLPA 4161, utilizes my interest in microbes and their important role in society. The course emphasizes guided student presentations, discussions and debates. Several field trips to view microbes in action are taken during the semester. The Department’s needs for instruction in phytobacteriology led me to offer courses in bacterial plant pathogens for some 15 years, which ended in 2004.
teaching activities
- PLPA-6820: Graduate Student Research Updates - Spring 2013
- PLPA-9900: Doctoral-Level Thesis Research - Spring 2013
- PLPA-6820: Graduate Student Research Updates - Fall 2012
- PLPA-6820: Graduate Student Research Updates - Spring 2012
- PLPA-9900: Doctoral-Level Thesis Research - Spring 2012
- PLPA-6820: Graduate Student Research Updates - Fall 2011
- PLPA-7980: Graduate Teaching Experience - Fall 2011
- PLPA-7990: Graduate-Level Thesis Research - Fall 2011
- PLPA-9900: Doctoral-Level Thesis Research - Fall 2011
Service
outreach overview
- An innovative outreach project aimed primarily at secondary school biology students in New York State is underway. It`s a collaboration with Cornell`s Institute for Biology Teaching (CIBT), which is supported by the Howard Hughes Medical Institute and the New York State Center for Advanced Technology. A DVD and a study guide were produced aimed at introducing secondary school students and their teachers to several important concepts: that bacteria can cause disease in plants; techniques for safe handling of bacterial plant pathogens; that specific genes are needed for disease-causing ability; bioinformatics strategies useful for annotation of a genome sequence, and the use of reverse genetics laboratory tests to assess gene function. The program was presented to a group of secondary school science teachers, who will test the materials and possibly suggest changes to the project for use in classrooms. The materials also will be made available on the internet. The secondary school outreach project is being revised and expanded for presentation to the agricultural community, especially Cooperative Extension Educators in the field and fruit growers. One of the major objectives is to foster better understanding by the target audiences of fundamental molecular biological research as applied to fruit crops and their pests and pathogens. Greater understanding of the relevance of molecular biological research, and its possible future benefit, likely will result in enhanced appreciation by the agricultural community and the general public.
- Since becoming involved in research on bacterial problems in onions, I have also functioned as an extension resource for the topic. For example, I address onion growers and others in meetings to provide research updates and address questions relative to onion diseases. I also visit farms and examine problems, make diagnoses, and advise growers and CCE staff on bacterial problems.
service to the profession
- Sigma Xi
- Organizing Committee, Fallen Leaf Lake Conference on Bacterial Virulence Mechanisms Committee Member - 1992
- Fire Blight Working Group Chairperson 1986 - 1989
- Organizing Committee, Fallen Leaf Lake Conference on Molecular Biology of Bacterial Plant Pathogens Committee Member - 1989
- Organizing Committee, Fallen Leaf Lake Conference on Erwinia Committee Member - 1987
- Biological Control Committee Committee Member 1984 - 1986
- Fire Blight Working Group Officer, Secretary 1983 - 1986
- Bacteriology Committee Committee Member 1977 - 1983
- Bacteriology Committee Chairperson 1980 - 1981
- Public Relations Committee Committee Member 1976 - 1979
- Mycotoxicology and Post-Harvest Pathology Committee Committee Member 1975 - 1978
- Executive Committee Committee Member 1972 - 1976
- Executive Committee Chairperson 1972 - 1976
reviewer or editor for
Background
education and training
- Ph.D. in Plant Pathology, University of California 1969
- B.S. in Agriculture; Biochemistry, Cornell University 1965
awards and honors
- Green Chemistry Award (Member of a team responsible for the discovery and development of harpin as an agricultural biochemical), 2001
- Honored Professor of Crop Genetics, 1991
- NYS CALS Faculty Travel Fellowship, 1989
- NYS CALS Faculty Travel Fellowship, 1979
- Society of Sigma Xi, 1968
- NDEA Graduate Fellow (1965-1968), 1965
- NSF Undergraduate Research Fellow (Summers, 1962-64), 1962
- American Men and Women in Science (Listed)
Other
college
- CALS
research keyword
- bacteriology
- disease etiology
- fire-blight
- fire-blight, erwinia, disease, bacteria, apple, pear,
- gene-expression
- gene-sequencing
- gene-silencing
- genomics
- host-specificity
- microbiology
- molecular-biology
- molecular-genetics
- onion decay
- phytobacteriology
- phytopathology
- plant-disease
- plant-microbe-biology
- plant-molecular-biology
- transgenic-plants
name prefix
- Dr.