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Perry, Keith Lloyd
Cornell Faculty Member
Positions
- Associate Professor, Plant Pathology and Plant-Microbe Biology (PPPMB), College of Agriculture and Life Sciences (CALS)
My training is in Plant Pathology, having worked and trained at U.C. Berkeley, U.C.Davis, Chevron Chemical Company, Cornell University, University of Adelaide (Waite Institute), and Purdue University. I worked for six years on bacterial plant pathogens, and for the past 18 years on plant viruses. A common thread in my work has been in the diagnosis of plant diseases and the detection of plant pathogens. Teaching responsibilities have included graduate courses in plant pathology and plant virology, an undergraduate laboratory course in molecular biological techniques, and a general interest microbiology course for undergraduates. For the past seven years, I have had administrative oversight of the foundation potato seed program for New York State.
Research Areas
- agricultural biotechnology
- biochemistry
- biotechnology
- crop management or crop science
- disease control
- entomology
- insects
- integrated pest management
- international agriculture
- molecular biology
- nanotechnology
- organic agriculture
- pathogens
- pest management
- plant pathology
Websites
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- Publications
- Teaching
- Service
- Background
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Affiliations
other Cornell affiliations
Research
research overview
- There are three foci in my work: i) oversight of the NY State Foundation Seed Potato Program and Cornell Uihlein Laboratory and Farm, ii) research on plant viruses and their aphid vector transmission, and iii) the diagnostic detection of plant viruses and other pathogens. My objectives in the potato program are: to ensure the successful operation and financial viability of the Uihlein Farm, to address the needs of the NY seed potato growers and industry, and to facilitate the development of golden nematode resistant potato varieties at Cornell for NY State. My primary area of research is the vector transmission of plant viruses. Aphids are the most common plant virus vector and my goal is to understand, at the molecular level, how an aphid is able to acquire virus from an infected plant and deliver it to a new site of infection. The specificity of interactions between a virus and its vector is determined to a large extent by the properties of the virus capsid protein. I have collaborated to obtain an atomic structure for cucumber mosaic virus and this structure is being used to understand how the surface architecture and physical properties of the virus affect and determine its vector transmission. A third major emphasis in my research is the development of multipathogen detection systems for diseases of solanaceous crops (potato, tomato, pepper, eggplant). In this cooperative project, nucleic-acid based methods are being utilized in a macroarray format for the detection of virus, fungi and oomycete pathogens. The same technology is being applied for the detection of grapevine viruses to facilitate the propagation of healthy nursery stocks in NY State
research activities
principal investigator on
- DEVELOPMENT, IMPLEMENTATION, AND OUTREACH FOR EMERGING TECHNOLOGIES OF PLANT PATHOGEN DETECTION awarded by NATIONAL INSTITUTE OF FOOD AND AGRICULTURE-USDA 2009 - 2012
- INTEGRATED RESEARCH AND EDUCATION RELATED TO DEVELOPMENT OF A UNIVERSAL PLANT VIRUS MICROARRAY awarded by USDA-ARS FRUIT LABORATORY 2010 - 2012
- MANAGEMENT OF VIRUS DISEASE IN POTATO AND GRAIN CROPS awarded by USDA ARS NORTH ATLANTIC AREA 2011 - 2014
- PRODUCTION OF FOUNDATION QUALITY SEED OF NEW USDA/ARS POTATO VARIETIES awarded by USDA-ARS FRUIT LABORATORY 2008 - 2013
- SEQUENCE SPECIFIC, NUCLEIC ACID SEPARATION MEDIA FOR PLANT PATHOGEN DIAGNOSTICS awarded by NATIONAL INSTITUTE OF FOOD AND AGRICULTURE-USDA 2011 - 2012
co-principal investigator on
- ACCELERATION OF THE DEVELOPMENT, RELEASE, AND ACCEPTANCE OF POTATO CULTIVARS RESISTANT TO THE GOLDEN NEMATODE awarded by ANIMAL/PLANT HLTH INSPEC SERV USDA 2012
- DEVELOPMENT AND RELEASE OF GOLDEN NEMATODE RESISTANT POTATOES awarded by AGRICULTURE RESEARCH SERVICE USDA (USDA-ARS) 2010 - 2015
- DNA NANOTECHNOLOGY-BASED DETECTION FOR MULTIPLEXED ON-SITE DIAGNOSIS OF PLANT PATHOGENS awarded by NATIONAL INSTITUTE OF FOOD AND AGRICULTURE-USDA 2011 - 2014
- GOLDEN NEMATODE RESEARCH PROJECT awarded by NYS DEPT OF AG & MARKETS 2008 - 2013
- POTATO BREEDING AND VARIETY DEVELOPMENT FOR IMPROVED QUALITY AND PEST RESISTANCE IN THE EASTERN UNITED STATES awarded by NATIONAL INSTITUTE OF FOOD AND AGRICULTURE-USDA 2011 - 2013
- POTATO BREEDING AND VARIETY DEVELOPMENT TO ENHANCE PEST RESISTANCE AND MARKETING OPPORTUNITIES IN THE EASTERN UNITED STATES awarded by NATIONAL INSTITUTE OF FOOD AND AGRICULTURE-USDA 2010 - 2012
area(s) of concentration/expertise
keywords
- Disease
- Grape
- Plant pathology
- Potato
- Vector
- Virus
submitted impact statement
- Integrated technologies for the rapid diagnosis of known and emerging plant pathogens
- Integrated technologies for the rapid diagnosis of known and emerging plant pathogens
- Integrated technologies for the rapid diagnosis of known and emerging plant pathogens
- Integrated technologies for the rapid diagnosis of known and emerging plant pathogens
Publications
individual publications
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academic article
- Complete Genome Sequence of a New Circular DNA Virus from Grapevine. Journal of Virology. 86:7715. 2012
- Encapsidation of DNA, a protein and a fluorophore into virus-like particles by the capsid protein of cucumber mosaic virus. Journal of General Virology. 93:1120-1126. 2012
- Genomic analysis of Grapevine leafroll associated virus-5 and related viruses.. Virus Research. 163:19-27. 2012
- Macroarray detection of grapevine leafroll-associated viruses.. Journal of Virological Methods. 183:161-169. 2012
- A natural M RNA reassortant arising from two species of plant- and insect-infecting bunyaviruses and comparison of its sequence and biological properties to parental species. Virology. 413:216-225. 2011
- Argonaute2 mediates RNA silencing anti-viral defences against Potato virus X in Arabidopsis. Plant Physiology. 156:1556-1564. 2011
- First report of Tomato chlorosis virus infecting tomato in Georgia. Plant Disease. 95:881. 2011
- Potato virus M in Bittersweet Nightshade (Solanum dulcamara L.) in New York State. Plant Disease. 95:619. 2011
- Transmission efficiency of Potato virus Y strains PVYO and PVYN-Wi by five aphid species. Plant Disease. 95:1279-1283. 2011
- A cucumber mosaic virus mutant that induces resistance to its aphid vector in tobacco. Phytopathology. 100. 2010
- A tospovirus new to North America: Virus detection and discovery through the use of a macroarray for viruses of solanaceous crops. Phytopathology. 100. 2010
- Alterations of capsid protein amino acid positions internal to virions of Cucumber mosaic virus disrupt nonpersistent virus transmission by aphids. Phytopathology. 100. 2010
- DNA microarray-based universal plant virus detection and identification. Phytopathology. 100. 2010
- First report of Groundnut ringspot virus infecting tomato in South Florida. Plant Health Progress. 2010
- The capsid protein of Cowpea chlorotic mottle virus is a determinant for vector transmission by a beetle. Journal of General Virology. 91:545-551. 2010
- Universal plant virus microarray development and validation. Phytopathology. 100. 2010
- The capsid protein of Cowpea chlorotic mottle virus is a determinant for vector transmission by a beetle. Phytopathology. 99. 2009
- Transmission efficiency of Potato virus Y strains PVYO and PVYN-Wi by five aphid species. Phytopathology. 99. 2009
- A multipathogen detection array for virus, viroid, fungal, and oomycete pathogens of solanaceous crops. Phytopathology. 98. 2008
- Emerging diversity in Potato virus Y poses new challenges for the U.S. potato industry. Phytopathology. 98. 2008
- Macroarray detection of eleven potato-infecting viruses and Potato spindle tuber viroid. Plant Disease. 92:730-740. 2008
- Macroarray detection of plant RNA viruses using randomly primed and amplified complementary DNAs from infected plants. Phytopathology. 97:119-127. 2007
- Potato aucuba mosaic virus in potato in New York State. Plant Dis. 91:1202. 2007
- Virus detection using array-based technologies. Phytopathology. 97. 2007
- Biological and serological properties of Potato Virus Y isolates in Northeastern US potatoes. Plant Disease. 90:559-566. 2006
- Compensatory capsid protein mutations in Cucumber mosaic virus confer systemic infectivity in squash (Cucurbita pepo). Journal of Virology. 80:7740-7743. 2006
- Marcy, a Chipping Variety with Resistance to Common Scab and the Golden Nematode. American Journal of Potato Research. 83:189-193. 2006
- Impatiens necrotic spot virus in greenhouse-grown potatoes in New York State. Plant Disease. 89:340. 2005
- A new potato virus in a new lineage of picorna-like viruses. Archives of Virology. 149:2141-2154. 2004
- Transmission of plant viruses by aphid vectors. Molecular Plant Pathology. 5:505-511. 2004
- A conserved capsid protein surface domain of Cucumber mosaic virus is essential for efficient aphid vector transmission. Journal of Virology. 76. 2002
- An antibody to the putative aphid recognition site on Cucumber mosaic virus recognizes pentons but not hexons. Journal of Virology. 76:12250-12258. 2002
- Transmissibility of field isolates of soybean viruses by Aphis glycines. Plant Disease. 86:1219-1222. 2002
- Aphid transmissibility of cucumber mosaic virus by Aphis gossypii correlates with viral accumulation and is affected by the presence of satellite RNA. Phytopathology. 90:1068-1072. 2000
- Cucumber mosaic virus mutants with altered physical properties and defective in aphid vector transmission. Virology. 276:395-403. 2000
- Identification and subgrouping of cucumber mosaic cucumoviruses with mouse monoclonal antibodies. Phytopathology. 90:615-620. 2000
- Registration of ‘Goldfield’ Wheat. Crop Science Journal. 40:581-582. 2000
- Registration of ‘Jay’ Spring Oat. Crop Science Journal. 40:569. 2000
- The structure of cucumber mosaic virus and comparison to cowpea chlorotic mottle virus. Journal of Virology. 74:7578-7586. 2000
- Yield effects of barley yellow dwarf virus in soft red winter wheat. Phytopathology. 90:1043-1048. 2000
- Cytological and molecular characterization of wheat lines with Thinopyrum intermedium chromosome additions substitutions and translocations resistant to barley yellow dwarf virus. Cytologia. 64:93-100. 1999
- Stability of the aphid transmission phenotype in cucumber mosaic virus. Plant Pathology. 48:388-394. 1999
- Amino acid changes in the coat protein of cucumber mosaic virus differentially affect transmission by the aphids Myzus persicae and Aphis gossypii. Virology. 242:204-210. 1998
- Identification of epitopes in cucumber mosaic virus using a phage-displayed random peptide library. Journal of General Virology. 79:3145-3153. 1998
- Level and spectrum of resistance to barley yellow dwarf virus in a wheat alien chromosomal substitution line. Phytopathology. 88:851-855. 1998
- Registration of barley yellow dwarf viruses resistant wheat germplasm line P29. Crop Science Journal. 37:1032-1033. 1997
- Mapping determinants in cucumber mosaic virus for transmission by Aphis gossypii. Virology. 205:591-595. 1994
- Specificity of replicase-mediated resistance to cucumber mosaic virus. Virology. 201:200-205. 1994
- A varied population of cucumber mosaic virus from peppers. Plant Pathology. 42:806-810. 1993
- Insect-mediated transmission of mixed and reassorted cucumovirus genomic RNAs. Journal of General Virology. 73:2105-2114. 1992
- Transcription of tomato ribosomal DNA and the organization of the intergenic spacer. Molecular and General Genetics. 221:102-112. 1990
- Cloning and regulation of Erwinia herbicola pigment genes. Journal of Bacteriology. 168:607-612. 1986
- Characterization of the replication and stability regions of Agrobacterium tumefaciens plasmid pTAR. Journal of Bacteriology. 157:739-745. 1984
- Characteristics of Ti plasmids from broad-host-range and ecologically specific biotype 2 and 3 strains of Agrobacterium tumefaciens. Journal of Bacteriology. 151:343-350. 1982
- Indoleacetic acid complementation and its relation to host range specifying genes on the Ti plasmid of Agrobacterium tumefaciens. Molecular and General Genetics. 188:425-432. 1982
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article
-
chapter
- Aphids - Non-persistent Transmission. Advances in Botanical Research. 1-19. 2002
- Cucumoviruses. Virus-Insect-Plant Interactions. 167-180. 2001
- Virus isolation, RNA extraction and location of coat protein genes: Luteovirus group. Plant Virology Protocols. 231-239. 1998
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conference paper
- Multiplex detection of viruses infecting grapevine using a randomly primed, RT-PCR/macroarray platform.. 2012 APS Annual Meeting. 2012
- Development of a macroarray for the detection of grapevine leafroll-associated viruses in grapevine. Proc. 30th Annual meeting of the American Society for Virology, July 16-20, Minneapolis Minnesota. 2011
- Membrane-based macroarrays for virus detection. BARD Workshop Proceedings - Microarrays and Next-Generation Sequencing for Detection and Identification of Plant Viruses. 2011
- A tospovirus new to North America: Virus detection and discovery through the use of a macroarray for viruses of solanaceous crops. BARD Workshop Proceedings - Microarrays and Next-Generation Sequencing for Detection and Identification of Plant Viruses. 2010
- Agrobacterium Ti plasmid indoleacetic acid gene is required for crown gall oncogenesis. Proceedings of the National Academy of Sciences USA. 2812-2816. 1982
-
report
- Groundnut ringspot virus detected in tomato in south Florida 2010
Teaching
teaching overview
- PLPA 6020 The Biology of Plant Pathogens
teaching activities
- PLPA-4020: Biology of Plant Pathogens - Spring 2013
- PLPA-7980: Graduate Teaching Experience - Spring 2013
- PLPA-7990: Graduate-Level Thesis Research - Spring 2013
- PLPA-8900: Master's Level Thesis Research - Spring 2013
- PLPA-9900: Doctoral-Level Thesis Research - Spring 2013
- PLPA-7980: Graduate Teaching Experience - Fall 2012
- PLPA-7990: Graduate-Level Thesis Research - Fall 2012
- PLPA-8900: Master's Level Thesis Research - Fall 2012
- PLPA-9900: Doctoral-Level Thesis Research - Fall 2012
- PLPA-7980: Graduate Teaching Experience - Spring 2012
- PLPA-7990: Graduate-Level Thesis Research - Spring 2012
- PLPA-8900: Master's Level Thesis Research - Spring 2012
- PLPA-9900: Doctoral-Level Thesis Research - Spring 2012
- BIOG-2990: Introduction to Research Methods in Biology - Fall 2011
- PLPA-7980: Graduate Teaching Experience - Fall 2011
- PLPA-7990: Graduate-Level Thesis Research - Fall 2011
- PLPA-8900: Master's-Level Thesis Research - Fall 2011
- PLPA-9900: Doctoral-Level Thesis Research - Fall 2011
Service
outreach overview
- Foundation seed potato production for New York State seed potato growers- My work in the potato program has a large service component and overlaps with my research program. Foundation potato seed is produced at the Uihlein Farm of Cornell University to address the needs of the NY seed potato growers and industry, and to facilitate the development of golden nematode resistant potato varieties at Cornell for NY State.
service to the profession
- USDA CSREES Multistate (MRF) project (WERA-89) ‘Potato Virus Disease Control’ Committee Member 2002 -
- Golden Nematode Technical Workgroup Committee Member 2001 -
- International Committee on the Taxonomy of Viruses Study Group on Luteoviruses Committee Member 1996 -
- American Society for Virology
- USDA Pest Management Alternatives (PMAP) program Research Review Panel 2011
- USDA CSREES Multistate (MRF) project (NC-502) ‘Soybean Aphid' Committee Member 2000 - 2002
reviewer or editor for
Background
education and training
- Ph.D. in Plant Pathology, Cornell University 1989
- University of California, Davis 1981
- B.A. in, University of California, Berkeley 1977
awards and honors
Other
college
- CALS
research keyword
- Disease
- Grape
- Plant pathology
- Potato
- Vector
- Virus
name prefix
- Dr.