Calcium as a second messenger in non-excitable cells
protein kinase C
ion channels in mast cells
Stimulus-response coupling in cells in the immune system and the role of Ca2+ in this process
Characterizing the immunoglobulin E receptor-activated signal transduction pathway in mucosal mast cells, and in particular the role of Ca2+ and protein kinase C
Measurements of intracellular Ca2+ and membrane potential using fluorescent probes and quantitative image-enhanced fluorescence microscopy
Identification of the exocytosis protein that acts as the Ca2+ sensor in mast cells
Indo-1 photometry and constant-potential amperometry are being used to monitor intracellular Ca2+ and serotonin secretion at the single-cell level, with high temporal resolution
Characterizing the relationship between release of Ca2+ from intracellular stores, ion channel activation, oscillations in cytoplasmic Ca2+ and the individual exocytotic events occurring in a single cell
calcium and cell activation
selected publications listing
Mahmoud, S.F. & Fewtrell, C. Microdomains of high calcium are not required for exocytosis in RBL-2H3 mucosal mast cells. J. Cell Biol.153:339-349 (2001).
Mulvaney, J.M., Zhang, T., Fewtrell, C. & Roberson, M.S. Calcium influx through L-type channels is required for selective activation of extracellular signal-regulated kinase by gonadotropin-releasing hormone. J. Biol. Chem.274: 29796-298014 (1999).
Kuchtey, J. & Fewtrell, C. Protein kinase C activator PMA reduces the Ca2+ response to antigen stimulation of adherent RBL-2H3 mucosal mast cells by inhibiting depletion of intracellular Ca2+ stores. J. Cell Physiol.181: 113-123 (1999).
Kim, T.D., Eddlestone, G.T., Mahmoud, S.F., Kuchtey, J. & Fewtrell, C. Correlating Ca2+ responses and secretion in individual RBL-2H3 mucosal mast cells. J. Biol. Chem.272: 31225-31229 (1997).
Chang, E.-Y., Szallasi, Z., Acs, P., Raizada, V., Wolfe, P.C., Fewtrell, C., Blumberg, P.M. & Rivera, J. Functional effects of overexpression of the protein kinase C isozymes -a, -b, -d, -e, and -h in the mast cell line RBL-2H3. J. Immunol.159: 2624-2632 (1997).
Wolfe, P.C., Chang, E-Y., Rivera, J. & Fewtrell, C. Differential effects of the protein kinase C activator PMA on calcium responses and secretion in adherent and suspended RBL-2H3 mucosal mast cells. J. Biol. Chem.271: 6658-6665 (1996).
Kuchtey, J. & Fewtrell, C. Subcloning the RBL-2H3 mucosal mast cell line reduces Ca2+ response heterogeneity at the singe-cell level. J. Cell. Physiol.166: 643-652 (1996).
Falcone, D. & Fewtrell, C. Ca2+-ATPase inhibitior, cyclopiazonic acid, releases Ca2+ from intracellular stores in RBL-2H3 mast cells and activates a Ca2+ influx pathway that is permeable to sodium and manganese. J. Cell. Physiol.164: 205-213 (1995).
Chandra, S., Fewtrell, C., Millard, P.J., Sandison, D.R., Webb, W.W. & Morrison, G.H. Imaging of total intracellular calcium and calcium influx and efflux in individual resting and stimulated tumor mast cells using ion microscopy. J. Biol. Chem.269: 15186-15194 (1994).
Weintraub, W.H., Cleveland-Wolfe, P. & Fewtrell, C. Paracrine Ca2+ signaling in vitro: Serotonin-mediated cell-cell communication in mast cell/smooth muscle cocultures. J. Cell. Physiol.160: 389-399 (1994).
Smith, P.A., Ashcroft, F.M. & Fewtrell, C.M.S. Permeation and gating properties of the L-type calcium channel in mouse pancreatic b cells. J. Gen. Physiol.101: 767-797 (1993).
Fewtrell, C. Ca2+ oscillations in non-excitable cells. Ann. Rev. Physiol.55: 427-454 (1993).
Cleveland, P.L., Millard, P.J., Showell, H.J. & Fewtrell, C.M.S. Tenidap: a novel inhibitor of calcium influx in a mast cell line. Cell Calcium14: 1-16 (1993).
Ashcroft, F.M., Williams, B.A., Smith, P.A. & Fewtrell, C. Ion channels involved in the regulation of nutrient-stimulated insulin secretion. Chapter 10 in: Nutrient Regulation of Insulin Secretion. (ed. Flatt, P.R.) pp.193-212. Portland Press, London (1992).
Ashcroft, F.M., Smith, P.A. & Fewtrell, C. L-type Ca-channel activity recorded at a low divalent cation concentration. Biomed. Res.12: Supp. 2, 61-63 (1991).
Mohr, F.C. & Fewtrell, C. The effect of mitochondrial inhibitors on calcium homeostasis in tumor mast cells. Am. J. Physiol. 258: C217-C226 (1990).
Marcotte, G.V., Millard, P.J. & Fewtrell, C. Release of calcium from intracellular stores in rat basophilic leukemia cells monitored with the fluorescent probe chlortetracycline. J. Cell. Physiol. 142: 78-88 (1990).