Highly proliferative, relatively undifferentiated canine osteosarcoma cell lines and a highly differentiated epithelial cell in which proliferation is developmentally regulated to study cell proliferation and differentiation
Established five canine osteosarcoma cell lines from independent animals with the goal of identifying the oncogenes, tumor suppressor genes, and signaling pathways that are aberrantly regulated and the mechanisms by which these mutant proteins and pathways control cellular proliferation and differentiation
Screening cDNA libraries to identify and study genes that regulate type 2 cell proliferation and differentiation
selected publications listing
Levine, R.A., Forest, T., and Smith, C. (2002) Tumor suppressor PTEN is mutated in canine osteosarcoma cell lines and tumors. Vet. Pathol. 39:372-378.
Levine, R. A. (2002) Overexpression of the sis oncogene in a canine osteosarcoma cell line. Vet. Pathol. 39:411-412.
Levine, R.A. and Fleischlii, M.A. (2000) Inactivation of p53 and retinoblastoma family pathways in canine osteosarcoma cell lines. Vet. Pathology 37:54-61.
Battle, T.E., Levine, R.A., and Yen, A. (2000) Retinoic acid-induced Blr1 expression promotes ERK2 activation and cell differentiation in HL-60 cells. Cell Res. 254:287-298.
Abdel-Ghany, M., Cheng, H., Levine, R. A., and Pauli, B. U. (1998) Truncated dipeptidyl peptidase IV is a potent anti-adhesion and anti-metastasis peptide for rat breast cancer cells. Invasion Metastasis 18:35-43.
Levine, R.A., Hopman, T., Guo, L., Chang, M-J., Johnson, N. (1998) Induction of retinoblastoma gene expression during terminal growth arrest of a conditionally immortalized fetal rat lung epithelial cell line and during fetal lung maturation. Exp. Cell Res. 239:264-276.
Gruber, A.D. and Levine, R.A. (1997) In situ assessment of mRNA accessibility in heterogeneous tissue samples using Elongation Factor-1a. Histochem. Cell Biol. 107:411-416.
Elble, R.C., Widom, J., Gruber, A.D., Abdel-Ghany, M., Levine, R.A., Goodwin, A., Cheng, H.-C., Pauli, B.U. (1997) Cloning and characterization of Lung-Endothelial Cell Adhesion Molecule-1 suggest it is an endothelial chloride channel. J. Biol. Chem. 272:27853-27861.
Joyce-Brady, M., Takahashi, Y., Oakes, S.M., Rishi, A., Levine, R.A., Kinlough, C.L., Hughey, R.P. (1994) Synthesis and release of amphipathic gamma-glutamyl transferase by the Pulmonary Alveolar Type 2 Cell: Its redistribution throughout the gas exchange portion of the lung indicates a new role for surfactant. J. Biol. Chem. 269:14219-14226.