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Tumbar, Tudorita
Cornell Faculty Member
Positions
- Associate Professor, Molecular Biology and Genetics (MBG), College of Agriculture and Life Sciences (CALS)
Tudorita Tumbar was born in Romania in 1970 and obtained her Bachelor degree in Biochemistry from University of Bucharest in 1993. She then came to the United States for her PhD work in Cell Biology at University of Illinois at Urbana-Champaign. Her post-doctoral training with Elaine Fuchs began at University of Chicago and continued at Rockefeller University. She is now leading her own research group in the Department of Molecular Biology and Genetics at Cornell University working on molecular mechanisms controlling cell fate of hair follicle stem cells.
Research Areas
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Affiliations
other Cornell affiliations
Research
research overview
- Our laboratory is interested in elucidating the basic cellular and molecular mechanisms implicated in cell fate choice and stem cell activity within tissues. We use the mouse as a model system. An important focus is on the control of nuclear function and structure including transcriptional networks, chromatin structure and dynamics, and DNA replication. Understanding how progenitor and differentiated cells function in normal tissue morphogenesis and homeostasis will reveal how deregulation of their precise control of growth and differentiation can lead to diseased tissues and cancer. Our laboratory focuses on adult stem cells and their interaction with their tissue of residence, and uses the mouse skin as a primary model system to address the general questions outlined above. Within the skin epithelia stem cells are thought to reside both in the outer epidermis and in a specialized area of the hair follicle called the bulge. The bulge is a stem cell niche thought to keep their potent resident cells in a differentiation and proliferation inhibited state. It allows external signals to selectively penetrate and instruct stem cells to migrate out and proliferate when they are needed: during the initiation of the hair follicle growth and in wounded, regenerating skin. The bulge area is marked by a profoundly quiescent population of cells, known to incorporate BrdU (bromodeoxyuridine) label in the newly synthesized DNA and retain this label preferentially relative to other cells in the skin, for extended periods of time during the animal adult life. These label retaining cells (LRCs) have been attributed stem cell potential for more than 20 years, not only in the hair follicle, but also in other tissues where they have been identified. We have devised a novel technique to isolate LRCs from tissues based on their GFP retention. A pulse and chase with histone H2B fused with GFP, with expression regulated by the tetracycline inducible system, allowed us to label and isolate live LRCs from the hair follicle bulge. Using a genomic approach a large number of factors preferentially expressed in bulge cells were identified. A significant fraction of these factors were likely involved in the cross-talk between bulge cells and the surrounding environment, suggesting a possible role for LRCs in organizing the stem cell niche.
research activities
principal investigator on
- CONTROL OF HAIR FOLLICLE STEM CELL PROLIFERATION AND EPITHELIAL SKIN TUMOROGENESIS BY CONCERTED RUNX1 AND CDKN1A IN ACTION awarded by NEW YORK STATE DEPARTMENT OF HEALTH 2009 - 2012
- EPIGENETIC REGULATION OF STEM CELL FATE ACQUISITION IN MOUSE SKIN awarded by INTERNATIONAL HUMAN FRONTIER SCIENCE PROGRAM ORGANIZATION 2011 - 2014
- MOLECULAR MECHANISMS OF CELL FATE DECISIONS IN HAIR FOLLICLE STEM CELLS awarded by NATL INST OF HEALTH DHHS 2010 - 2015
keywords
- hair follicle stem cells
submitted impact statement
Publications
individual publications
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academic article
- New Insights into Mechanisms of Stem Cell Daughter Fate Determination in Regenerative Tissues. International reviews in cell and molecular biology. 300:1-50. 2013
- New insights into the role of Runx1 in epithelial stem cell biology and pathology. Journal of Cell Biochemistry. 114:985-93. 2013
- Defining a tissue stem cell-driven Runx1/Stat3 signalling axis in epithelial cancer. EMBO Journal. 31:4124-39. 2012
- Hairy tale of signaling in hair follicle development and cycling. Seminars in cell and developmental Biology. 23:906-16. 2012
- Ontogeny and Homeostasis of Adult Epithelial Skin Stem Cells. Stem cells reviews and reports. 8:561-576. 2012
- Runx1 modulates adult hair follicle stem cell emergence and maintenance from distinct embryonic skin compartments . Journal of Cell Biology. 193:235-50. 2011
- Runx1 Directly Promotes Proliferation of Hair Follicle Stem Cells and Epithelial Tumor Formation in Mouse Skin. Molecular and Cellular Biology. 30:2518-36. 2010
- Stem cell dynamics in mouse hair follicles: a story from cell division counting and single cell lineage tracing. Cell Cycle. 9:1504-1510. 2010
- Acute Myeloid Leukemia Factor 1 Controls Developmental but not Injury Driven Activation of Hair Follicle Stem Cells. Development. 135:1059-1068. 2008
- Quantitative Proliferation Dynamics and Random Chromosome Segregation of Hair Follicle Stem Cells. EMBO Journal. 27:1309-20. 2008
- Epithelial skin stem cells. Methods in Enzymology. 419:73-99. 2006
- Defining the Ephitelial Stem Cell Niche in Skin. Science. 303:359-363. 2004
- Socializing with the neighbors: stem cells and their niche. Cell. 116:769-778. 2004
- Interphase movements of a DNA chromosome region modulated by VP16 transcriptional activator. Nature Cell Biology. 3:134-139. 2001
- Visualization of gene activity in living cells. Nature Cell Biology. 2:871-878. 2000
- Large-scale chromatin structure and function. Current Opinion in Cell Biology. 11:307-311. 1999
- Large-scale chromatin unfolding and remodeling induced by VP16 acidic activation domain. Journal of Cell Biology. 145:1341-1354. 1999
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chapter
- Linking large-scale chromatin structure with nuclear function. Chromatin Structure and Gene Expression. 300-321. 2000
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report
presentations
featured in archived article
speaker at Cornell event
Teaching
teaching overview
- Tudorita Tumbar teaches on class on Stem Cell Biology (BIOMG445), with the objective to give students a general understanding of the concepts, terminology, and methodology currently used in stem cell biology. The possibility of using stem cells in various therapies will constitute a theme of the research papers. These are meant to offer students a realistic view of the current status of the field with respect to bringing basic research to the level of clinical application. This is an upper undergraduate and graduate level class that will allow students to acquire or practice skills in oral research presentations, as well as grant proposal writing and creative thinking. This class is not meant as a comprehensive survey of the stem cell field.
teaching activities
- BIOMG-4450: Stem Cell Biology: Basic Science and Clinical Applications - Spring 2013
- TOX-8900: Master's Thesis and Research - Spring 2013
- TOX-9900: Doctoral Thesis and Research - Spring 2013
- TOX-8900: Master's Thesis and Research - Fall 2012
- TOX-9900: Doctoral Thesis and Research - Fall 2012
- BIOMG-7800: Current Topics in Genetics and Development - Spring 2012
- TOX-8900: Master's Thesis and Research - Spring 2012
- TOX-9900: Doctoral Thesis and Research - Spring 2012
- TOX-8900: Master's Thesis and Research - Fall 2011
- TOX-9900: Doctoral Thesis and Research - Fall 2011
Service
service to the profession
- International Society for Stem Cell Research Member 2009 -
- American Society for Cell Biology Member 2008 -
- American Association for the Advancement of Science Member 2007 -
Background
education and training
- Ph.D. in Cell Biology, University of Illinois 2000
- University of Bucharest 1993
awards and honors
Other
college
- CALS
research keyword
- hair follicle stem cells
name prefix
- Dr.