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Knipple, Douglas C
Cornell Faculty Member
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Positions
- Associate Professor, Entomology at Geneva
Douglas C. Knipple is Associate Professor of Insect Molecular Genetics in the Department of Entomology at the New York State Agricultural Experiment Station in Geneva. He received a B.S. degree in Biology from Hobart College in 1976 and a Ph.D. in Genetics from Cornell University in 1983. He did postdoctoral studies at the Max-Planck Institut for Developmental Biology in TŸbingen, Germany from 1983-1985. He joined the Cornell faculty in 1985.
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Affiliations
Research
research overview
- Major areas of investigation have focused on ion channels and neurotransmitter receptors of the insect nervous system, enzymes used by lepidopteran inects for sex pheromone biosynthesis, and RNA binding proteins that play essential roles in the embryonic development of the codling moth,
area(s) of concentration/expertise
- Anthropod Behavior
- Biochemistry
- Biochemistry and Molecular and Cell Biology
- Bioinformatics
- Fruit, Arthropod Pest Biology & Control
- Genetics (Drosophila)
- Insect Developmental Biology
- Insect Molecular Genetics
- Insect Phermone Perception and Behavior
- Insect Physiology/Biochemistry
- Insecticide Resistance and Toxicology
keywords
- RNA binding proteins
- acyl-CoA desaturases
- biochemistry
- embryonic development
- entomology
- gene family evolution
- insecticide target sites
- integral membrane proteins
- lepidopteran pheromone biosynthesis
- ligand-gated chloride channels
- male reproductive biology
- molecular genetics
- target site resistance ion channels voltage-sensitive sodium channel neurotransmitter receptors
submitted impact statement
- Practical applications of cloned insect desaturase genes in crop protection
- Practical applications of cloned insect desaturase genes in crop protection
- Practical Applications of Cloned Insect Desaturase Genes in Crop Protection
- Practical Applications of Cloned Insect Desaturase Genes in Crop Protection
Publications
individual publications
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academic article
- Prospects for the use of transgenic approaches to improve the efficacy of the Sterile Insect Technique (SIT) for control of the codling moth Cydia pomonella Crop Protection. Crop Protection. 44:142-146. 2013
- Recent Advances in RNA Interference Research in Insects: Implications for Future Insect Pest Management Strategies. Crop Protection. 45:36-40. 2013
- The ligand-gated chloride channel gene family of Drosophila melanogaster. Pesticide Biochemistry and Physiology. 97:140-148. 2010
- The ligand-gated chloride channel gene family of Drosophila melanogaster. Pesticide Biochemistry and Physiology. doi:1016/j.pestbp.2009.09.002:9. 2009
- An abundant acyl-CoA (Δ9) desaturase transcript in pheromone glands of the cabbage moth, Mamestra brassicae, encodes a catalytically inactive protein. Insect Biochemistry and Molecular Biology. 38:581-595. 2008
- cis-3-Hexenal production in tobacco is stimulated by 16-carbon monounsaturated fatty acids. Phytochemistry. 65:159-168. 2004
- Ethanol tolerance in the yeast Saccharomyces cerevisiae is dependent on cellular oleic acid content. Applied and Environmental Microbiology. 69:1499-1503. 2003
- Multiple acyl-CoA desaturase-encoding transcripts in pheromone glands of Helicoverpa assulta, the oriental tobacco budworm. Insect Biochemistry and Molecular Biology. 33:609-622. 2003
- The molecular biology of knockdown resistance. Insect Biochemistry and Molecular Biology. 33:563-577. 2003
- Developmental regulation of alternative exon usage in the house fly Vssc1 sodium channel gene. Invertebrate Neuroscience. 4:125-133. 2002
- Evolution of the integral membrane desaturase gene family in moths and flies. Genetics. 162:1737-1752. 2002
- Effects of the sap of the common oleander Nerium indicum (Apocyanacea) on male fertility and spermatogenesis in the oriental tobacco budworm Helicoverpa assulta (Lepidoptera, Noctuidae). Journal of Experimental Biology. 204:3935-3942. 2001
- Structural and functional conservation and divergence among acyl-CoA desaturase-encoding genes of two noctuid species, the corn earworm, Helicoverpa zea, and the cabbage looper, Trichoplusia ni. Insect Biochemistry and Molecular Biology. 31:949-964. 2001
- A delta 9 desaturase gene with a different substrate specificity is responsible for the cuticular diene hydrocarbon polymorphism in Drosophila melanogaster. Proceedings at the National Academy of Science USA. 97:9449-9454. 2000
- Cloning and functional expression of a cDNA encoding a metabolic acyl-CoA delta 9-desaturase of the cabbage looper moth, Trichoplusia ni. Insect Biochemistry and Molecular Biology. 29:435-443. 1999
- Knockdown resistance to DDT and pyrethroids in the house fly (Musca domestica L.): from genetic trait to molecular mechanism. Annals Entomol. Soc. Amer. 92:909-915. 1999
- Mutations in the house fly Vssc1 sodium channel gene associated with super-kdr resistance abolish the pyrethroid sensitivity of Vssc1/tipE sodium channels expressed in Xenopus oocytes. Insect Biochemistry and Molecular Biology. 29:185-194. 1999
- Baculovirus expression of an insect gene that encodes multiple neuropeptides. Insect Biochemistry and Molecular Biology. 28:239-249. 1998
- Cloning and characterization of a cDNA encoding a pheromone gland-specific acyl-CoA ∆11-desaturase of the cabbage looper moth, Trichoplusia ni. Proceedings of the National Academy of Sciences (PNAS). 95:15287-15292. 1998
- Cloning and functional expression of a cDNA encoding a pheromone gland-specific acyl-CoA Δ11-desaturase of the cabbage looper moth, Trichoplusia ni. Proceedings at the National Academy of Science USA. 95:15287-15292. 1998
- Expression of a gene that encodes pheromone biosynthesis activating neuropeptide in the central nervous system of corn earworm, Helicoverpa zea. Insect Biochemistry and Molecular Biology. 28:373-385. 1998
- The L1014F point mutation in the house fly Vssc1 sodium channel gene confers knockdown resistance to pyrethroids. Insect Biochemistry and Molecular Biology. 27:807-812. 1997
- Characterization of voltage-sensitive sodium channel gene coding sequences from insecticide-susceptible and knockdown-resistant house fly strains. Insect Biochemistry and Molecular Biology. 26:319-326. 1996
- PCR-based homology probing reveals a family of GABA receptor-like genes in Drosophila melanogaster. Insect Biochemistry and Molecular Biology. 24:363-371. 1994
- Structural organization of the Helicoverpa zea gene encoding the precursor protein for PBAN and other neuropeptides. Proceedings at the National Academy of Science USA. 91:6506-6510. 1994
- Tight genetic linkage between the kdr insecticide resistance trait and a voltage-sensitive sodium channel gene in the house fly. Proceedings at the National Academy of Science USA. 91:2483-2487. 1994
- Characterization of a putative gamma-aminobutyric acid (GABA) receptor beta subunit gene from Drosophila melanogaster. Biochemical and Biophysical Research Communications. 193:474-482. 1993
- Expression of a scorpion insectotoxin peptide in bacteria, yeast and plant. Gene. 116:165-172. 1992
- Structure, expression and hormonal control of genes from the mosquito, Aedes aegypti, which encode proteins similar to the vitelline membrane proteins of Drosophila melanogaster. Developmental Biology. 155:558-568. 1992
- Development of functionally competent cabbage looper moth sex pheromone glands. Insect Biochemistry. 21:573-581. 1991
- PCR-based phylogenetic walking: Isolation of sodium channel gene sequences from insects and mites by DNA amplification. Insect Biochemistry. 21:689-696. 1991
- PCR-generated conspecific sodium channel gene probe for the house fly homologue of the para locus of Drosophila melanogaster. Archives of Insect Biochemistry and Physiology. 16:45-53. 1991
- Prolonged exposure to GABA activates GABA-gated chloride channels in the presence of channel-blocking convulsants. Comp Biochem Physiol C. 99:397-402. 1991
- Molecular cloning and analysis of chromosomal region 26A of Drosophila melanogaster. Molec. Gen. Genetics. 226:241-249. 1990
- Knockdown resistance to dichlorodiphenyl-trichloroethane and pyrethroid insecticides in the napts mutant of Drosophila melanogaster is correlated with reduced neuronal sensitivity. Archives of Insect Biochemistry and Physiology. 10:293-302. 1989
- Molecular neurobiology: implications for insecticide action and resistance. Pesticide Science. 26:359-374. 1989
- Versatile plasmid vectors for the construction, analysis, and heat-inducible expression of hybrid genes in eukaryotic cells. Nucleic Acids Research. 16:7748. 1988
- Purification and characterization of β-galactosidase-1 from Drosophila melanogaster. Insect Biochem. 17:1163-1171. 1987
- Molecular analysis of Krüppel, a segmentation gene of Drosophila melanogaster. Cold Spring Harbor Symp. Quant. Biol. 50:465-474. 1986
- An early role of maternal mRNA in establishing the dorso-ventral pattern in pelle mutant Drosophila embryos. Developmental Biology. 110:238-246. 1985
- Production of phenocopies by Krüppel antisense RNA injection into Drosophila embryos. Nature. 313:703-706. 1985
- Spatial and temporal patterns of Krüppel gene expression in early Drosophila embryos. Nature. 317:40-44. 1985
- Cytogenic mapping and isolation of mutations of the β-Gal-1 locus of Drosophila melanogaster. Molecular and General Genetics. 198:75-83. 1984
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booksection
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chapter
- Molecular Biological Investigations of Pheromone Desaturases. Insect Pheromone Biochemistry and Molecular Biology. 81-106. 2003
- In vivo synthesis in yeast of insect sex pheromone precursors: An alternative synthetic route for the production of environmentally benign insect control agents. Green Chemical Syntheses and Processes. 33-42. 2000
- Actions of insecticides on sodium channels: multiple target sites and site-specific resistance. Molecular Action of Insecticides on Ion Channels. 97-108. 1995
- Structural and functional characterization of insect genes encoding ligand-gated chloride channel subunits. Molecular Action of Insecticides on Ion Channels. 205-215. 1995
- Molecular genetic approach to the study of target site resistance to pyrethroids and DDT in insects. Biotechnology in Crop Protection. 199-214. 1988
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conference paper
- Isolation of insect genes coding for voltage-sensitive sodium channels and ligand-gated chloride channels by PCR-based homology probing. Neurotox '91: Molecular Basis of Drug and Pesticide Action. 271-283. 1992
- Neuropharmacology and molecular genetics of nerve insensitivity resistance to pyrethroids. Molecular basis of drug & pesticide action: proceedings of Neurotox '88, molecular basis of drug & pesticide action, University of Nottingham, England, 10-15 April 1988. 553-561. 1988
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document part
- The ligand-gated chloride channel gene family of Drosophila. 1901. 2000
- Functional analysis of resistance-associated mutations in house fly sodium channels. 1821. 1998
- A point mutation in a house fly sodium channel gene confers insecticide resistance. 910. 1997
- Action of pyrethroid insecticides on house fly sodium channels expressed in Xenopus oocytes. 910. 1997
- Characterization of sodium channel genes from insecticide-susceptible and insecticide-resistant house flies. 1824. 1995
- Characterization of a putative gamma-aminobutyric acid (GABA) receptor beta subunit gene from Drosophila melanogaster. 478. 1993
- Characterization of an unusual member of the ligand-gated chloride channel gene family of Drosophila melanogaster. 489. 1993
- Construction of a cDNA library from cabbage looper moth sex pheromone glands. 368. 1990
- Development of probes for the isolation of presumptive voltage-sensitive sodium channel genes from insects 1990
- Isolation of presumptive voltage-sensitive sodium channel genes from insects. 346-347. 1990
Teaching
teaching overview
- This is an upper level course in insect development offered for the first time in Fall 2010. This course provides an in-depth treatment of the morphogenetic events of insect development, underlying mechanisms and the genetic programs controlling them. Much of its content focuses on the development of the fruit fly, Drosophila melanogaster, with particular emphasis on the establishment of the basic insect body plan, organogenesis and metamorphosis. However, because many aspects of Drosophila development are highly specialized, in cases where more ancestral processes occur in other insect taxa, these will also be addressed. The primary niche of this course in the context of the CALS curriculum is as an upper level core course in the Entomology undergraduate major. In addition, students from other majors in the Biological Sciences, such as Genetics and Development, and graduate students may find this course of interest.
teaching activities
- ENTOM-8900: Master's Level Thesis Research - Spring 2013
- ENTOM-9900: Doctoral Level Thesis Research - Spring 2013
- BIOMG-7800: Current Topics in Genetics and Development - Fall 2012
- ENTOM-9900: Doctoral Level Thesis Research - Fall 2012
- ENTOM-8900: Master's Level Thesis Research - Spring 2012
- ENTOM-9900: Doctoral Level Thesis Research - Spring 2012
- BIOMG-4710: Insect Development - Fall 2011
- ENTOM-4710: Insect Development - Fall 2011
- ENTOM-9900: Doctoral-Level Thesis Research - Fall 2011
Service
service to the profession
- AMERICAN CHEMICAL SOCIETY Member 1999 - 2013
reviewer or editor for
Background
education and training
- Ph.D. in Genetics, Cornell University 1983
- B.A. in Biology, Hobart College 1976
Other
college
- CALS
research keyword
- RNA binding proteins
- acyl-CoA desaturases
- biochemistry
- embryonic development
- entomology
- gene family evolution
- insecticide target sites
- integral membrane proteins
- lepidopteran pheromone biosynthesis
- ligand-gated chloride channels
- male reproductive biology
- molecular genetics
- target site resistance ion channels voltage-sensitive sodium channel neurotransmitter receptors
name prefix
- Professor